EPIC Code

LAB342
CD4

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Flow Cytometry

Collect

6.0 mL Dark Green no gel sodium heparin (Flow Cytometry testing) and 4.0 mL Purple EDTA (CBC testing) drawn at the same time.

Minimum Volume:  1.0 mL in each tube.

Note:  A CBC with differential will automatically be performed and billed with this order. 

Pediatric Collection

At least 0.5 mL each in Dark Green top no gel sodium heparin (Flow Cytometry testing) and Purple EDTA (CBC testing), drawn at the same time.

 

Unacceptable Conditions

  • Specimens older than specified stability
  • Clotted specimen
  • Improperly stored specimen
  • Hemolyzed specimen
  • Incorrect container
  • Insufficient sample volume
  • Sample not properly identified

Storage/Transport Temperature

Internal:  Deliver to lab immediately.
Offsite: Do not centrifuge.  Deliver to lab immediately at ambient temperature.

Performed

Daily

Stability (from collection to initiation)

Ambient:  24 hours
Refrigerated:  Unacceptable
Frozen:  Unacceptable

Remarks

CD4 Helper T Cells panel requires an absolute lymphocyte count.  A complete blood count (CBC) and differential (DIFF) is automatically ordered when the CD4 Helper T Cells panel order is placed. CPT code 85025 or 85027 and 85007 will be billed as needed.

Performed

Daily

Methodology

Flow Cytometry

Reported

Within 24 hours of receipt in the laboratory

Synonyms

  • CD4 ABSOLUTE COUNT
  • CD4 HELPER T CELL

EPIC Code

LAB342
CD4

Reference Interval

Component Sex From Age To Age Normal Low Normal High Units
HELPER T CELLS: %CD3+CD4+ M/F 18 150 25 62 %
HELPER T CELLS: ABS CD3+CD4+ M/F 18 150 425 1860 /uL

CPT Codes

CD4 Helper T Cells: 86361
CBC with differential: 85025 or 85027 and 85007

Collection

EPIC Code

LAB342
CD4

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Flow Cytometry

Collect

6.0 mL Dark Green no gel sodium heparin (Flow Cytometry testing) and 4.0 mL Purple EDTA (CBC testing) drawn at the same time.

Minimum Volume:  1.0 mL in each tube.

Note:  A CBC with differential will automatically be performed and billed with this order. 

Pediatric Collection

At least 0.5 mL each in Dark Green top no gel sodium heparin (Flow Cytometry testing) and Purple EDTA (CBC testing), drawn at the same time.

 

Unacceptable Conditions

  • Specimens older than specified stability
  • Clotted specimen
  • Improperly stored specimen
  • Hemolyzed specimen
  • Incorrect container
  • Insufficient sample volume
  • Sample not properly identified

Storage/Transport Temperature

Internal:  Deliver to lab immediately.
Offsite: Do not centrifuge.  Deliver to lab immediately at ambient temperature.

Performed

Daily

Stability (from collection to initiation)

Ambient:  24 hours
Refrigerated:  Unacceptable
Frozen:  Unacceptable

Remarks

CD4 Helper T Cells panel requires an absolute lymphocyte count.  A complete blood count (CBC) and differential (DIFF) is automatically ordered when the CD4 Helper T Cells panel order is placed. CPT code 85025 or 85027 and 85007 will be billed as needed.

Ordering

Performed

Daily

Methodology

Flow Cytometry

Reported

Within 24 hours of receipt in the laboratory

Synonyms

  • CD4 ABSOLUTE COUNT
  • CD4 HELPER T CELL

EPIC Code

LAB342
CD4

Result Interpretation

Reference Interval

Component Sex From Age To Age Normal Low Normal High Units
HELPER T CELLS: %CD3+CD4+ M/F 18 150 25 62 %
HELPER T CELLS: ABS CD3+CD4+ M/F 18 150 425 1860 /uL
Administrative

CPT Codes

CD4 Helper T Cells: 86361
CBC with differential: 85025 or 85027 and 85007

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