LAB4081
National Jewish Health Advanced Diagnostic Laboratories
3 6.0mL Dark Green top tubes (15 mL preferred)
Minimum volume: 10 mL
Samples must arrive at the testing laboratory within 24 hours of collection and should be collected Monday thru NOON on Thursday. Samples are NOT acceptable on Fridays.
This test should only be ordered on immunocompromised patients with previously INDETERMINATE Qunatiferon Gold test results.
Do NOT Centrifuge. Store/transport at room temperature.
Samples received at the testing laboratory after 1200 on Thursdays or on Fridays.
Frozen, clotted, hemolyzed, spun down, citrated anticoagulant.
Maintain and transport specimens at room temperature (18-22 C).
Monday-Thursday
24 hours at room temperature.
Monday-Thursday
Elispot
In 48 hours.
This test should only be ordered on immunocompromised patients with previously INDETERMINATE Qunatiferon Gold test results.
LAB4081
Negative
The T-SPOT.TB test is based on the measurement of a cell mediated response to Mycobacterium tuberculosis-specific antigens (ESAT-6 and CFP-10). Peripheral blood mononuclear cells (PBMC) are separated from whole blood and incubated in plates coated with antiinterferongamma (IFNg) antibodies with these antigens for 16-24 hours. Following incubation, IFNg-producing cells are detected usinga second anti- IFNg antibody, an enzyme linked conjugate, and substrate. Controls include unstimulated cells (Nil control) andphytohemagglutinin stimulated cells (Mitogen control). A test is considered positive or borderline when IFNg- spot forming units (SFU) produced in response to either of the TB antigens is significantly greater than the Nil control (TB-Nil; positive = > 8 SFU, borderline = 5, 6 or 7 SFU), and the Mitogen control has a robust IFNg response. The T-Spot.TB result is indeterminate if the Nil control is high (>10 SFU) or the mitogen control is low (< 20 SFU). Although the test is reported qualitatively, IFNg SFU for the individual test c
1.The T-Spot.TB detects infections due to M. tuberculosis complex (M. tuberculosis, M. bovis and M. africanum). BCG strains and
nontuberculous mycobacteria with the exception of M. marinum, M. kansasii, M. szulgai and M. gordonae do not express ESAT-6 and CFP-10 proteins. Therefore, patients either vaccinated with BCG or infected with most nontuberculous mycobacterial strains should test negative.
T-Spot.TB results should always be interpreted in conjunction with clinical and other relevant laboratory findings.
1 Guidelines for Using the QuantiFERON®-TB Gold Test for Detecting Mycobacterium tuberculosis Infection, United States. Centers for Disease Control and Prevention. Morbidity and Mortality Weekly Report (MMWR). December 16, 2005 / 54(RR15);49-55
onditions are also reported as recommended by the Centers for Disease Control and Prevention.
86481
| Collection |
LAB4081
National Jewish Health Advanced Diagnostic Laboratories
3 6.0mL Dark Green top tubes (15 mL preferred)
Minimum volume: 10 mL
Samples must arrive at the testing laboratory within 24 hours of collection and should be collected Monday thru NOON on Thursday. Samples are NOT acceptable on Fridays.
This test should only be ordered on immunocompromised patients with previously INDETERMINATE Qunatiferon Gold test results.
Do NOT Centrifuge. Store/transport at room temperature.
Samples received at the testing laboratory after 1200 on Thursdays or on Fridays.
Frozen, clotted, hemolyzed, spun down, citrated anticoagulant.
Maintain and transport specimens at room temperature (18-22 C).
Monday-Thursday
24 hours at room temperature.
| Ordering |
Monday-Thursday
Elispot
In 48 hours.
This test should only be ordered on immunocompromised patients with previously INDETERMINATE Qunatiferon Gold test results.
LAB4081
| Result Interpretation |
Negative
The T-SPOT.TB test is based on the measurement of a cell mediated response to Mycobacterium tuberculosis-specific antigens (ESAT-6 and CFP-10). Peripheral blood mononuclear cells (PBMC) are separated from whole blood and incubated in plates coated with antiinterferongamma (IFNg) antibodies with these antigens for 16-24 hours. Following incubation, IFNg-producing cells are detected usinga second anti- IFNg antibody, an enzyme linked conjugate, and substrate. Controls include unstimulated cells (Nil control) andphytohemagglutinin stimulated cells (Mitogen control). A test is considered positive or borderline when IFNg- spot forming units (SFU) produced in response to either of the TB antigens is significantly greater than the Nil control (TB-Nil; positive = > 8 SFU, borderline = 5, 6 or 7 SFU), and the Mitogen control has a robust IFNg response. The T-Spot.TB result is indeterminate if the Nil control is high (>10 SFU) or the mitogen control is low (< 20 SFU). Although the test is reported qualitatively, IFNg SFU for the individual test c
1.The T-Spot.TB detects infections due to M. tuberculosis complex (M. tuberculosis, M. bovis and M. africanum). BCG strains and
nontuberculous mycobacteria with the exception of M. marinum, M. kansasii, M. szulgai and M. gordonae do not express ESAT-6 and CFP-10 proteins. Therefore, patients either vaccinated with BCG or infected with most nontuberculous mycobacterial strains should test negative.
T-Spot.TB results should always be interpreted in conjunction with clinical and other relevant laboratory findings.
1 Guidelines for Using the QuantiFERON®-TB Gold Test for Detecting Mycobacterium tuberculosis Infection, United States. Centers for Disease Control and Prevention. Morbidity and Mortality Weekly Report (MMWR). December 16, 2005 / 54(RR15);49-55
onditions are also reported as recommended by the Centers for Disease Control and Prevention.
| Administrative |
86481
