Heme samples : one 5 mL blood in EDTA (lav top), OR one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells)
Formalin-Fixed, paraffin-embedded tissue (FFPE) in microtainer: 3-5 10uM slices or >100K cells
Formalin-Fixed, paraffin-embedded tissue (FFPE) slides: 10 unstained slides with slices of 10uM, and an associated H&E slide with tumor cells marked. Include surgical pathology report with tumor cellularity estimate in marked region.
Fresh tissue: min 0.1mL
Frozen tissue: min 100mg or 0.5cc / 0.5 cm3
Body Fluids: >200K cells
Fine needle aspirate slides: Minimum volume is dependent on the tumor size and cellularity. Unfixed, ethanol fixed, or H&E stained – Soak slide in xylene to remove coverslip and dry before transporting to the lab.
Storage/Transport Temperature
20-25°C for several hours acceptable Protect FFPE samples from heat exposure, especially in summer months. Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium. Frozen samples should be sent on dry ice.
Unacceptable Conditions
Specimens fixed/processed in alternative fixatives. Samples without a tumor cellularity estimate
Heme samples : one 5 mL blood in EDTA (lav top), OR one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells)
Formalin-Fixed, paraffin-embedded tissue (FFPE) in microtainer: 3-5 10uM slices or >100K cells
Formalin-Fixed, paraffin-embedded tissue (FFPE) slides: 10 unstained slides with slices of 10uM, and an associated H&E slide with tumor cells marked. Include surgical pathology report with tumor cellularity estimate in marked region.
Fresh tissue: min 0.1mL
Frozen tissue: min 100mg or 0.5cc / 0.5 cm3
Body Fluids: >200K cells
Fine needle aspirate slides: Minimum volume is dependent on the tumor size and cellularity. Unfixed, ethanol fixed, or H&E stained – Soak slide in xylene to remove coverslip and dry before transporting to the lab.
Storage/Transport Temperature
20-25°C for several hours acceptable Protect FFPE samples from heat exposure, especially in summer months. Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium. Frozen samples should be sent on dry ice.
Unacceptable Conditions
Specimens fixed/processed in alternative fixatives. Samples without a tumor cellularity estimate
