Ordering Recommendations

The pitted red blood cell count is a semiquantitative method using phase microscopy to assess the splenic reticuloendothelial function. In individuals with a dysfunctional or absent spleen the membrane of erythrocytes appears to demonstrate the formation of "pits" or crater-like depressions in RBC membranes.  

Determination of pitted RBC count offers a simple means for clinical evaluation of splenic function in patients with sickle cell disease. 

Routine TAT

1-5 days 

Methodology

Phase microscopy

Performed

Mon - Fri: 8 am - 5 pm

Performing Lab

BCH Oak Hemoglobin Reference Lab

Patient Preparation

none

Collect

Collect 1 drop of whole blood in a 1% Glutaraldehyde tube. Contact the Lab for collection tubes in advance. 

Sample Type

Whole Blood

Amount to Collect

1 Drop

Preferred Volume

1 Drop

Minimum Volume

1 Drop

Rejection Criteria

Unlabeled or mislabeled specimens

Unacceptable Conditions

None

Stability (from collection to initiation)

The prefilled 1% glutaraldehyde tubes must be kept refrigerated prior to collection.

After collection, the samples are stable when stored in the refrigerator.

Please ship the samples to the lab overnight with an ice pack or send via courier.

Avoid freezing the samples.

Storage/Transport Temperature

Refrigerated prior to collection and shipped with an ice pack.

Performed

Mon - Fri: 8 am - 5 pm

Remarks

Fresh blood

Storage/Transport Temperature

Refrigerated prior to collection and shipped with an ice pack.

Units

Percentage 

Reference Interval

< 3%

Performing Lab

BCH Oak Hemoglobin Reference Lab

LDT or modified Test

This assay was developed and validated by the Hemoglobinopathy Reference Laboratory according to CLIA requirements. These assays have not been cleared or approved by the US Food and Drug Administration.

CPT Codes

85999

Amount to Collect

1 Drop

Collect

Collect 1 drop of whole blood in a 1% Glutaraldehyde tube. Contact the Lab for collection tubes in advance. 

CPT Codes

85999

LDT or modified Test

This assay was developed and validated by the Hemoglobinopathy Reference Laboratory according to CLIA requirements. These assays have not been cleared or approved by the US Food and Drug Administration.

Methodology

Phase microscopy

Minimum Volume

1 Drop

Ordering Recommendations

The pitted red blood cell count is a semiquantitative method using phase microscopy to assess the splenic reticuloendothelial function. In individuals with a dysfunctional or absent spleen the membrane of erythrocytes appears to demonstrate the formation of "pits" or crater-like depressions in RBC membranes.  

Determination of pitted RBC count offers a simple means for clinical evaluation of splenic function in patients with sickle cell disease. 

Patient Preparation

none

Performed

Mon - Fri: 8 am - 5 pm

Performing Lab

BCH Oak Hemoglobin Reference Lab

Preferred Volume

1 Drop

Reference Interval

< 3%

Rejection Criteria

Unlabeled or mislabeled specimens

Remarks

Fresh blood

Routine TAT

1-5 days 

Sample Type

Whole Blood

Stability (from collection to initiation)

The prefilled 1% glutaraldehyde tubes must be kept refrigerated prior to collection.

After collection, the samples are stable when stored in the refrigerator.

Please ship the samples to the lab overnight with an ice pack or send via courier.

Avoid freezing the samples.

Storage/Transport Temperature

Refrigerated prior to collection and shipped with an ice pack.

Unacceptable Conditions

None

Units

Percentage 
Ordering

Ordering Recommendations

The pitted red blood cell count is a semiquantitative method using phase microscopy to assess the splenic reticuloendothelial function. In individuals with a dysfunctional or absent spleen the membrane of erythrocytes appears to demonstrate the formation of "pits" or crater-like depressions in RBC membranes.  

Determination of pitted RBC count offers a simple means for clinical evaluation of splenic function in patients with sickle cell disease. 

Routine TAT

1-5 days 

Methodology

Phase microscopy

Performed

Mon - Fri: 8 am - 5 pm

Performing Lab

BCH Oak Hemoglobin Reference Lab
Collection

Patient Preparation

none

Collect

Collect 1 drop of whole blood in a 1% Glutaraldehyde tube. Contact the Lab for collection tubes in advance. 

Sample Type

Whole Blood

Amount to Collect

1 Drop

Preferred Volume

1 Drop

Minimum Volume

1 Drop

Rejection Criteria

Unlabeled or mislabeled specimens

Unacceptable Conditions

None

Stability (from collection to initiation)

The prefilled 1% glutaraldehyde tubes must be kept refrigerated prior to collection.

After collection, the samples are stable when stored in the refrigerator.

Please ship the samples to the lab overnight with an ice pack or send via courier.

Avoid freezing the samples.

Storage/Transport Temperature

Refrigerated prior to collection and shipped with an ice pack.

Performed

Mon - Fri: 8 am - 5 pm

Remarks

Fresh blood
Processing

Storage/Transport Temperature

Refrigerated prior to collection and shipped with an ice pack.
Result Interpretation

Units

Percentage 

Reference Interval

< 3%

Performing Lab

BCH Oak Hemoglobin Reference Lab

LDT or modified Test

This assay was developed and validated by the Hemoglobinopathy Reference Laboratory according to CLIA requirements. These assays have not been cleared or approved by the US Food and Drug Administration.
Administrative

CPT Codes

85999
Complete View

Amount to Collect

1 Drop

Collect

Collect 1 drop of whole blood in a 1% Glutaraldehyde tube. Contact the Lab for collection tubes in advance. 

CPT Codes

85999

LDT or modified Test

This assay was developed and validated by the Hemoglobinopathy Reference Laboratory according to CLIA requirements. These assays have not been cleared or approved by the US Food and Drug Administration.

Methodology

Phase microscopy

Minimum Volume

1 Drop

Ordering Recommendations

The pitted red blood cell count is a semiquantitative method using phase microscopy to assess the splenic reticuloendothelial function. In individuals with a dysfunctional or absent spleen the membrane of erythrocytes appears to demonstrate the formation of "pits" or crater-like depressions in RBC membranes.  

Determination of pitted RBC count offers a simple means for clinical evaluation of splenic function in patients with sickle cell disease. 

Patient Preparation

none

Performed

Mon - Fri: 8 am - 5 pm

Performing Lab

BCH Oak Hemoglobin Reference Lab

Preferred Volume

1 Drop

Reference Interval

< 3%

Rejection Criteria

Unlabeled or mislabeled specimens

Remarks

Fresh blood

Routine TAT

1-5 days 

Sample Type

Whole Blood

Stability (from collection to initiation)

The prefilled 1% glutaraldehyde tubes must be kept refrigerated prior to collection.

After collection, the samples are stable when stored in the refrigerator.

Please ship the samples to the lab overnight with an ice pack or send via courier.

Avoid freezing the samples.

Storage/Transport Temperature

Refrigerated prior to collection and shipped with an ice pack.

Unacceptable Conditions

None

Units

Percentage 

Location Info

Performing Lab
BCH Oak Hemoglobin Reference Lab

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