Collection Container

Collect

3 mL Serum or Plasma, gold (SST) or green (PPT) top tube
Minimum volume: 0.2 mL

Storage/Transport Temperature

Room Temperature

Stability (from collection to initiation)

HUP
Room Temp: 24 hours
Refrigerated: 7 days at 2-8C

PAH
RT 24hrs, 2-8C 7days

Unacceptable Conditions

Gross hemolysis

Processing - HUP

Load the specimen in the automated track. If the specimen has to be manually centrifuged, spin the tube for 7-10 minutes at 1000-1300 X G. Ensure proper separation.

Processing - PAH

Spin 7-10 minutes at 3500 rpms. Bring specimen to Chemistry

Reference Interval

0.00-0.27 mmol/L

Clinical Significance

beta-Hydroxybutyrate, as well as blood glucose determination in diabetics, is used to evaluate the severity of diabetic coma and is essential in the exclusion of hyperosmolar non-ketotic diabetic coma. Moreover, the insulin requirement is often based on the extent of the existing hyperketonemia shown by the blood levels of beta-Hydroxybutyrate, and is therefore extremely important in assessment of ketosis.

CPT Codes

82010

LOINC

  • 6873-4
Collection & Processing

Collection Container

Collect

3 mL Serum or Plasma, gold (SST) or green (PPT) top tube
Minimum volume: 0.2 mL

Storage/Transport Temperature

Room Temperature

Stability (from collection to initiation)

HUP
Room Temp: 24 hours
Refrigerated: 7 days at 2-8C

PAH
RT 24hrs, 2-8C 7days

Unacceptable Conditions

Gross hemolysis
CR&P Information

Processing - HUP

Load the specimen in the automated track. If the specimen has to be manually centrifuged, spin the tube for 7-10 minutes at 1000-1300 X G. Ensure proper separation.

Processing - PAH

Spin 7-10 minutes at 3500 rpms. Bring specimen to Chemistry
Result Interpretation

Reference Interval

0.00-0.27 mmol/L

Clinical Significance

beta-Hydroxybutyrate, as well as blood glucose determination in diabetics, is used to evaluate the severity of diabetic coma and is essential in the exclusion of hyperosmolar non-ketotic diabetic coma. Moreover, the insulin requirement is often based on the extent of the existing hyperketonemia shown by the blood levels of beta-Hydroxybutyrate, and is therefore extremely important in assessment of ketosis.
Testing Updates
Billing Codes

CPT Codes

82010

LOINC

  • 6873-4

Ordering Information

Cerner Orderable
BHB
Penn Chart Orderable
Beta Hydroxybutyric Acid (aka BHB); BOH
Performing Lab
Chemistry
Performed
Sunday-Saturday
Reported
HUP & PAH
Routine: 4 hours
STAT: 1 hour
Methodology
beta-Hydroxybutyrate dehydrogenase enzyme spectrophotometry
Synonyms
  • BHB
  • Ketones, Serum