The Influenza and RSV by nucleic acid test is intended as an aid in the diagnosis of influenza and respiratory syncytial virus infections in conjunction with clinical and epidemiological risk factors.
This test provides increased sensitivity and specificity over rapid antigen tests and should especially be ordered when community prevalence is low.
Orderable SIM Number(s)
INFLUENZA A/B & RSV NAT, AMPLIVIED (LAB123031)
Synonyms
RSV
Flu
PCR
Flu A
Flu B
Respiratory Syncytial Virus
LABRSV
LAB123031
Collect
Nasal swab (NS) or Nasopharyngeal swab (NP) in 3.0 mL viral transport medium.
Collection procedure:
NP: Insert the sterile swab into either nostril, keeping the swab near the septum floor of the nose while gently pushing the swab into the posterior nasopharynx. Rotate the swab by firmly brushing against the nasopharynx several times.
NS: Insert a dry swab into a nostril. Rotate the swab against the inside of the nostril for 3 seconds while applying pressure with a finger to the outside of the nostril. Repeat on the other nostril with the same swab.
Place swab in UTM immediately and break the swab at the indicated break line and cap the tube.
Storage/Transport Temperature
Specimens must be transported at 2–8°C
Unacceptable Conditions
Swabs not submitted in approved liquid (dry swabs)
Swabs in saline.
Stability (from collection to initiation)
Room temperature (15–30°C) for up to 48 hours
Refrigerated (2–8°C) up to 7 days
Frozen (-80°C) and undergo 1 freeze thaw/cycle
Reference Interval
Normal
Influenza A: Negative
Influenza B: Negative
RSV: Negative
Abnormal
Influenza A: Positive
Influenza B: Positive
RSV: Positive
Interpretive Data
The Influenza A, B and RSV nucleic acid amplification test is an automated in vitro diagnostic real time RT-PCR test for the simultaneous qualitative detection and differentiation of RNA from Flu A, Flu B, and RSV. The primers and probes in the test are designed to amplify and detect unique sequences in the following: influenza A matrix (M), influenza A basic polymerase (PB2), influenza A acidic protein (PA), influenza B matrix (M), influenza B nonstructural protein (NS), and the RSV A and RSV B nucleocapsid.
Negative results do not preclude influenza or RSV infection and should not be used as the sole basis for treatment or other patient management decisions.
This test has not been evaluated for monitoring treatment of infection.
CPT Codes
87631
Billable SIM Number(s)
3068763101
LOINC
48509-4
Overview
Ordering Recommendations
The Influenza and RSV by nucleic acid test is intended as an aid in the diagnosis of influenza and respiratory syncytial virus infections in conjunction with clinical and epidemiological risk factors.
This test provides increased sensitivity and specificity over rapid antigen tests and should especially be ordered when community prevalence is low.
Orderable SIM Number(s)
INFLUENZA A/B & RSV NAT, AMPLIVIED (LAB123031)
Synonyms
RSV
Flu
PCR
Flu A
Flu B
Respiratory Syncytial Virus
LABRSV
LAB123031
Specimen
Collect
Nasal swab (NS) or Nasopharyngeal swab (NP) in 3.0 mL viral transport medium.
Collection procedure:
NP: Insert the sterile swab into either nostril, keeping the swab near the septum floor of the nose while gently pushing the swab into the posterior nasopharynx. Rotate the swab by firmly brushing against the nasopharynx several times.
NS: Insert a dry swab into a nostril. Rotate the swab against the inside of the nostril for 3 seconds while applying pressure with a finger to the outside of the nostril. Repeat on the other nostril with the same swab.
Place swab in UTM immediately and break the swab at the indicated break line and cap the tube.
Storage/Transport Temperature
Specimens must be transported at 2–8°C
Unacceptable Conditions
Swabs not submitted in approved liquid (dry swabs)
Swabs in saline.
Stability (from collection to initiation)
Room temperature (15–30°C) for up to 48 hours
Refrigerated (2–8°C) up to 7 days
Frozen (-80°C) and undergo 1 freeze thaw/cycle
Interpretive
Reference Interval
Normal
Influenza A: Negative
Influenza B: Negative
RSV: Negative
Abnormal
Influenza A: Positive
Influenza B: Positive
RSV: Positive
Interpretive Data
The Influenza A, B and RSV nucleic acid amplification test is an automated in vitro diagnostic real time RT-PCR test for the simultaneous qualitative detection and differentiation of RNA from Flu A, Flu B, and RSV. The primers and probes in the test are designed to amplify and detect unique sequences in the following: influenza A matrix (M), influenza A basic polymerase (PB2), influenza A acidic protein (PA), influenza B matrix (M), influenza B nonstructural protein (NS), and the RSV A and RSV B nucleocapsid.
Negative results do not preclude influenza or RSV infection and should not be used as the sole basis for treatment or other patient management decisions.
This test has not been evaluated for monitoring treatment of infection.
