Ordering Recommendations

Aids in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is decreased in low-risk populations. May be used in persons who have received the bacille Calmette-Guérin (BCG) vaccine.

Collect

QuantiFERON (R)-TB Gold Plus 1-tube (ARUP Supply #54015) available online through eSupply using ARUP Connect(TM) or contact ARUP Client Services at 800-522-2787. For collection and transport instructions, refer to QuantiFERON under Special Handling at https://www.aruplab.com/testing/quantiferon#collection. NOTE: The specimen must be submitted in the ARUP-provided collection tube due to the requirements of the laboratory automation.

Specimen Container

Specimen Preparation

Transport 6 mL whole blood. (Min: 5 mL).

Storage/Transport Temperature

Refrigerated. Must be collected and shipped directly to ARUP the same calendar day. 
 **Must arrive at Deaconess Midtown Lab by (2:00 pm CST)**

Specimens arriving after 2:00 pm will not make the daily ARUP pickup and will need to be recollected.

Unacceptable Conditions

Clotted specimens.

Remarks

Do not collect or ship on holidays or the day before holidays.

Stability (from collection to initiation)

Ambient: 3 hours; Refrigerated: 48 hours; Frozen: Unacceptable

Notes

If the stability requirements cannot be met, please refer to ARUP test code 3017562, QuantiFERON-TB Gold Plus, 4-Tube.

Referral Lab

Laboratories.

Ordering Recommendations

Aids in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is decreased in low-risk populations. May be used in persons who have received the bacille Calmette-Guérin (BCG) vaccine.

EPIC Order Code

LAB3373

Sunquest Code

QTBGS

Reference Lab Number

3000400

Synonyms

  • QFT-TB; QFT-TB Plus
  • QFT-G & M. tuberculosis
  • QFT & M TB
  • QuantiFERON-TB Gold Plus & Mycobacterium tuberculosis Antibody
  • QuantiFERON Mitogen and NIL
  • QFT-G & M. tuberculosis
  • QFT & M TB
  • Latent Tuberculosis Infection (LTBI)
  • Interferon-Gamma Release Assay for Tuberculosis
  • QuantiFERON-TB Gold Plus & Mycobacterium tuberculosis Antibody
  • QuantiFERON-TB Gold In Tube
  • Quantiferon Mitogen and NIL
  • QFT-TB Plus
  • QFT-TB
  • LAB3373
  • QTBGS
  • QFT-G & M. tuberculosis
  • QuantiFERON-TB Gold Plus, 1-Tube

CPT Codes

86480

LOINC

  • 71774-4
  • 71776-9
  • 64084-7
  • 88517-8
  • 71773-6

Reference Lab Number

3000400

Reference Interval

Components
Reference Interval
QuantiFERON Mitogen minus NIL
No Reference Interval

QuantiFERON NIL
No Reference Interval

Quantiferon Plus TB1 minus NIL
0.34 IU/mL or less

Quantiferon Plus TB2 minus NIL
0.34 IU/mL or less


Interpretive Data

Interferon gamma release is measured for specimens from each of the four collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values: NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. An overall Negative result does not completely rule out TB infection.

A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection -- United States, 2010 (http://www.cdc.gov/mmwr/preview/mmwrhtml/rr5905a1.htm), for more information concerning test performance in low-prevalence populations and use in occupational screening.

Collection

Ordering Recommendations

Aids in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is decreased in low-risk populations. May be used in persons who have received the bacille Calmette-Guérin (BCG) vaccine.

Collect

QuantiFERON (R)-TB Gold Plus 1-tube (ARUP Supply #54015) available online through eSupply using ARUP Connect(TM) or contact ARUP Client Services at 800-522-2787. For collection and transport instructions, refer to QuantiFERON under Special Handling at https://www.aruplab.com/testing/quantiferon#collection. NOTE: The specimen must be submitted in the ARUP-provided collection tube due to the requirements of the laboratory automation.

Specimen Container

Specimen Preparation

Transport 6 mL whole blood. (Min: 5 mL).

Storage/Transport Temperature

Refrigerated. Must be collected and shipped directly to ARUP the same calendar day. 
 **Must arrive at Deaconess Midtown Lab by (2:00 pm CST)**

Specimens arriving after 2:00 pm will not make the daily ARUP pickup and will need to be recollected.

Unacceptable Conditions

Clotted specimens.

Remarks

Do not collect or ship on holidays or the day before holidays.

Stability (from collection to initiation)

Ambient: 3 hours; Refrigerated: 48 hours; Frozen: Unacceptable

Notes

If the stability requirements cannot be met, please refer to ARUP test code 3017562, QuantiFERON-TB Gold Plus, 4-Tube.

Referral Lab

Laboratories.
Ordering

Ordering Recommendations

Aids in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is decreased in low-risk populations. May be used in persons who have received the bacille Calmette-Guérin (BCG) vaccine.

EPIC Order Code

LAB3373

Sunquest Code

QTBGS

Reference Lab Number

3000400

Synonyms

  • QFT-TB; QFT-TB Plus
  • QFT-G & M. tuberculosis
  • QFT & M TB
  • QuantiFERON-TB Gold Plus & Mycobacterium tuberculosis Antibody
  • QuantiFERON Mitogen and NIL
  • QFT-G & M. tuberculosis
  • QFT & M TB
  • Latent Tuberculosis Infection (LTBI)
  • Interferon-Gamma Release Assay for Tuberculosis
  • QuantiFERON-TB Gold Plus & Mycobacterium tuberculosis Antibody
  • QuantiFERON-TB Gold In Tube
  • Quantiferon Mitogen and NIL
  • QFT-TB Plus
  • QFT-TB
  • LAB3373
  • QTBGS
  • QFT-G & M. tuberculosis
  • QuantiFERON-TB Gold Plus, 1-Tube
Fees & Codes

CPT Codes

86480

LOINC

  • 71774-4
  • 71776-9
  • 64084-7
  • 88517-8
  • 71773-6

Reference Lab Number

3000400
Clinical & Interpretive

Reference Interval

Components
Reference Interval
QuantiFERON Mitogen minus NIL
No Reference Interval

QuantiFERON NIL
No Reference Interval

Quantiferon Plus TB1 minus NIL
0.34 IU/mL or less

Quantiferon Plus TB2 minus NIL
0.34 IU/mL or less


Interpretive Data

Interferon gamma release is measured for specimens from each of the four collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values: NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. An overall Negative result does not completely rule out TB infection.

A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection -- United States, 2010 (http://www.cdc.gov/mmwr/preview/mmwrhtml/rr5905a1.htm), for more information concerning test performance in low-prevalence populations and use in occupational screening.

Lab Use Only

Test Information

Performing Lab
ARUP

Samples collected Mon-Thurs till 11 a.m.
Methodology
Semi-Quantitative Chemiluminescent Immunoassay (CLIA) / Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)
Performed
Sun-Sat
Reported
1-4 days
Additional Technical Information
  • Time Sensitive

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