Ordering Instructions

MTB/RIF PCR performed in IDDL. LAB8286 will automatically order AFB Culture and AFB Stain to be performed at ARUP.

Special Instructions

See Acid-Fast Bacilli (AFB) Culture and Smear, Non-Blood (LAB877) for culture/smear details.

Test Includes

Note: Rifampin resistance (rpoB gene) by PCR will only be reported when MTB Complex DNA is detected.

Reflex Testing

Rifampicin (rpoB gene) resistance by PCR will be reflexed for positive tests for MTB.

Turnaround Time

Performed 24/7

Use

This test is FDA approved only for assessing isolation requirements for patients who are admitted to or potentially admitted to airborne precautions (negative air pressure). This test is NOT diagnostic for MTB. Cultures must also be ordered according to possible Mycobacterium tuberculosis protocols. Rifampicin (rpoB gene) resistance by PCR will be reflexed for positive tests for MTB. These results may guide treatment; however, culture with susceptibility testing is required to confirm the rifampicin results. A negative rifampicin resistance screen by Xpert MTB-RIF indicates likely susceptibility but NOT definitive susceptibility. Further testing is required.

Specimen Type

Expectorated Sputum

Collection Containers

Sterile Plastic Leakproof Container

Collection Instructions

Recommend early morning sputum collection. The MTBRIFPCR can be requested once on the first sputum collected. If not ordered on the first sputum, it may be added once to a subsequent sputum.

Collection Volume

7-13 mL for MTB/RIF PCR with AFB culture and stain

  • 2-3 mL for MTBRIF
  • 5-10 mL for AFB culture and stain 

(MTB/RIF will be canceled if AFB culture and stain are not ordered or sample is QNS for both MTB/RIF and AFB culture and stain)

Minimum Collection Volume

2.0 mL for MTB/RIF PCR with AFB culture and stain

  • 1.0 mL minimum for MTBRIF
  • 1.0 mL for AFB culture and stain 

(MTB/RIF will be canceled if AFB culture and stain are not ordered or sample is QNS for both MTB/RIF and AFB culture and stain)

Specimen Preparation

Do not process. Send immediately to laboratory.

Unacceptable Conditions

Samples with obvious food particles or other solid particulate matter are not acceptable for testing.

Shipping Instructions

Send immediately to laboratory.

Stability (from collection to initiation)

Specimen can be stored up to 7 days at refrigerated temperature.

Containers

Sterile Plastic Leakproof Container

Reference Interval

Not detected.

Contraindications

The Xpert MTB/RIF assay is not indicated for use with sputum samples from patients being treated with antituberculosis drugs either to determine bacteriologic cure or to monitor response to therapy. Assay interference may be observed in the present of Lidocaine (>20% v/v), mucin (>1.5% w/v), Ethambutol (>5 ug/mL), Guaifenesin (>2.5 mg/mL), Phenylephrine (25% v/v), or tea tree oil (>0.008% v/v) The performance of the Xpert MTB/RIF assay has not been evaluated with samples from pediatric patients.

CPT Disclaimer

The Current Procedural Terminology (CPT) Codes published in the M Health Fairview Test Directory are based on American Medical Association (AMA) guidelines and are provided for informational purposes only. CPT codes are provided only as guidance to assist clients with billing. CPT coding is the responsibility of the billing party. M Health Fairview Laboratories does not assume responsibility for billing errors due to reliance on the CPT codes listed in this Test Directory. Charges may vary due to reflexing, susceptibilities, specimen source, patient age, methodology requirements, etc..

Patient Price Inquiries

Requester Contact Information
Patient and UMP/FV Care Team Fairview Consumer Line at 612-672-1048
MRL Outreach Client dept-mrl-business-assessment@fairview.org
Research research@fairview.org

CPT Codes

CPT Qty HC Hospital PR Clinic Note
87564 1 3008756401 8756401 HC M TUBERCULOSIS RIFAMPIN RESISTANCE AMB PROBE

Methodology

Real-time polymerase chain reaction (PCR)

MRL Test Build

Test Name Component Required Description Type LOINC
LAB8286     Mycobacterium tuberculosis Complex Detection and Rifampin Resistance by PCR with AFB Culture and AFB Stain Orderable  
  1230010684   MTB Complex DNA Result 13956-8
  1230010685   Rifampin Resistance Result 46244-0
 

Contact

For questions regarding the test code Interface Map, please contact DEPT-LAB-CLIENT-INTERFACE@fairview.org
Ordering

Ordering Instructions

MTB/RIF PCR performed in IDDL. LAB8286 will automatically order AFB Culture and AFB Stain to be performed at ARUP.

Special Instructions

See Acid-Fast Bacilli (AFB) Culture and Smear, Non-Blood (LAB877) for culture/smear details.

Test Includes

Note: Rifampin resistance (rpoB gene) by PCR will only be reported when MTB Complex DNA is detected.

Reflex Testing

Rifampicin (rpoB gene) resistance by PCR will be reflexed for positive tests for MTB.

Turnaround Time

Performed 24/7

Use

This test is FDA approved only for assessing isolation requirements for patients who are admitted to or potentially admitted to airborne precautions (negative air pressure). This test is NOT diagnostic for MTB. Cultures must also be ordered according to possible Mycobacterium tuberculosis protocols. Rifampicin (rpoB gene) resistance by PCR will be reflexed for positive tests for MTB. These results may guide treatment; however, culture with susceptibility testing is required to confirm the rifampicin results. A negative rifampicin resistance screen by Xpert MTB-RIF indicates likely susceptibility but NOT definitive susceptibility. Further testing is required.
Collection & Processing

Specimen Type

Expectorated Sputum

Collection Containers

Sterile Plastic Leakproof Container

Collection Instructions

Recommend early morning sputum collection. The MTBRIFPCR can be requested once on the first sputum collected. If not ordered on the first sputum, it may be added once to a subsequent sputum.

Collection Volume

7-13 mL for MTB/RIF PCR with AFB culture and stain

  • 2-3 mL for MTBRIF
  • 5-10 mL for AFB culture and stain 

(MTB/RIF will be canceled if AFB culture and stain are not ordered or sample is QNS for both MTB/RIF and AFB culture and stain)

Minimum Collection Volume

2.0 mL for MTB/RIF PCR with AFB culture and stain

  • 1.0 mL minimum for MTBRIF
  • 1.0 mL for AFB culture and stain 

(MTB/RIF will be canceled if AFB culture and stain are not ordered or sample is QNS for both MTB/RIF and AFB culture and stain)

Specimen Preparation

Do not process. Send immediately to laboratory.

Unacceptable Conditions

Samples with obvious food particles or other solid particulate matter are not acceptable for testing.

Shipping Instructions

Send immediately to laboratory.

Stability (from collection to initiation)

Specimen can be stored up to 7 days at refrigerated temperature.

Containers

Containers

Sterile Plastic Leakproof Container

Result Interpretation

Reference Interval

Not detected.

Contraindications

The Xpert MTB/RIF assay is not indicated for use with sputum samples from patients being treated with antituberculosis drugs either to determine bacteriologic cure or to monitor response to therapy. Assay interference may be observed in the present of Lidocaine (>20% v/v), mucin (>1.5% w/v), Ethambutol (>5 ug/mL), Guaifenesin (>2.5 mg/mL), Phenylephrine (25% v/v), or tea tree oil (>0.008% v/v) The performance of the Xpert MTB/RIF assay has not been evaluated with samples from pediatric patients.
Administrative

CPT Disclaimer

The Current Procedural Terminology (CPT) Codes published in the M Health Fairview Test Directory are based on American Medical Association (AMA) guidelines and are provided for informational purposes only. CPT codes are provided only as guidance to assist clients with billing. CPT coding is the responsibility of the billing party. M Health Fairview Laboratories does not assume responsibility for billing errors due to reliance on the CPT codes listed in this Test Directory. Charges may vary due to reflexing, susceptibilities, specimen source, patient age, methodology requirements, etc..

Patient Price Inquiries

Requester Contact Information
Patient and UMP/FV Care Team Fairview Consumer Line at 612-672-1048
MRL Outreach Client dept-mrl-business-assessment@fairview.org
Research research@fairview.org

CPT Codes

CPT Qty HC Hospital PR Clinic Note
87564 1 3008756401 8756401 HC M TUBERCULOSIS RIFAMPIN RESISTANCE AMB PROBE

Methodology

Real-time polymerase chain reaction (PCR)
Interface Mapping

MRL Test Build

Test Name Component Required Description Type LOINC
LAB8286     Mycobacterium tuberculosis Complex Detection and Rifampin Resistance by PCR with AFB Culture and AFB Stain Orderable  
  1230010684   MTB Complex DNA Result 13956-8
  1230010685   Rifampin Resistance Result 46244-0
 

Contact

For questions regarding the test code Interface Map, please contact DEPT-LAB-CLIENT-INTERFACE@fairview.org
Private Details

Test Detail

Processing Key



See Processing Key "Special" Definitions document for *SPECIAL* definition.
Abbreviation
MTBRIFPCR
Performing Lab
UMMC East Bank
Department
Infectious Diseases Diagnostic Laboratory
Test Search Categories
  • Infectious Diseases

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