Special Instructions

It is recommended that HIV testing be discussed with the patient prior to collection of the specimen. Written patient consent for HIV testing is not required.

Reflex Testing

See Interpretive Data below.

Turnaround Time

Performed 24/7

Interpretive Data

The testing algorithm begins with the Roche Elecsys HIV Duo electrochemiluminescence immunoassay that simultaneously detects both antibody to human immunodeficiency virus (HIV-1 and HIV-2) and HIV-1 p24 antigen on the Roche Cobas instrument, performed at the East Bank campus. If the patient specimen is repeatedly reactive with the screening assay, it will be tested using the HIV Geenius (BioRad) HIV-1/2 immunoassay, which can differentiate between HIV-1 and HIV-2 antibodies. If the differentiation assay is also reactive, the presence of HIV-1 or HIV-2 antibodies is established. Specimens non-reactive with the HIV Geenius are reported as indeterminate and sent for HIV-1 RNA PCR testing performed by the Infectious Disease Diagnostic Laboratory. If HIV-2 is indeterminate, it is recommended to recollect and order testing for HIV-2 RNA Quantitation. If both HIV-1 and HIV-2 are positive, additional testing is required to rule out dual infection. The HIV Geenius CPT code is 86701 and 86702 and the Epic Code is LAB6328. The HIV Geenius will only be ordered by the Laboratory. The HIV-1 RNA Quantitation test code is LAB878 and the CPT code is 87536. HIV-2 RNA Quantitation is a send-out test performed by University of Washington Medical Center. Use the LAB4909 Laboratory Miscellaneous Order to order.

Associated Links

HIV Diagnostic Algorithm

Specimen Type

Blood

Collection Containers

10 mL Purple EDTA Tube
Alternate Containers: Green (lithium heparin, no gel), Green (lithium heparin, gel), Purple (EDTA), Red (no gel), Red or gold (gel)
 

Only for Blood and Body Fluid Exposure workups ordered via LAB704: 3 mL Purple (EDTA)

Collection Volume

9 mL

Minimum Collection Volume

5 mL

Only for Blood and Body Fluid Exposure workups ordered via LAB704: 3 mL

Note: this volume will not allow for any recommended reflex testing to occur

Specimen Preparation

Separate from cells within 24 hours of collection. 10 mL purple EDTA container recommended. If an alternate container is used the sample will not reflex to HIV 1 PCR testing.
Non-gel tubes: transfer serum/plasma to approved aliquot container.
Optimal testing volume: 2.5 mL serum/plasma; Minimum testing volume:1.55 mL

Unacceptable Conditions

Gross hemolysis or heat-inactivated specimens

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

After separation from cells:
Room temperature: 7 days
Refrigerated (preferred): 28 days
Frozen: 90 days (samples may be frozen up to 5 times)

Associated Links

HIV Diagnostic Algorithm

Containers

10 mL Purple EDTA Tube

Alternate Containers

Green (Lithium Heparin, No Gel)

Green (Lithium Heparin, Gel)

Purple (EDTA)

Red (No Gel)

Red or Gold (Gel)

Interpretive Data

The testing algorithm begins with the Roche Elecsys HIV Duo electrochemiluminescence immunoassay that simultaneously detects both antibody to human immunodeficiency virus (HIV-1 and HIV-2) and HIV-1 p24 antigen on the Roche Cobas instrument, performed at the East Bank campus. If the patient specimen is repeatedly reactive with the screening assay, it will be tested using the HIV Geenius (BioRad) HIV-1/2 immunoassay, which can differentiate between HIV-1 and HIV-2 antibodies. If the differentiation assay is also reactive, the presence of HIV-1 or HIV-2 antibodies is established. Specimens non-reactive with the HIV Geenius are reported as indeterminate and sent for HIV-1 RNA PCR testing performed by the Infectious Disease Diagnostic Laboratory. If HIV-2 is indeterminate, it is recommended to recollect and order testing for HIV-2 RNA Quantitation. If both HIV-1 and HIV-2 are positive, additional testing is required to rule out dual infection. The HIV Geenius CPT code is 86701 and 86702 and the Epic Code is LAB6328. The HIV Geenius will only be ordered by the Laboratory. The HIV-1 RNA Quantitation test code is LAB878 and the CPT code is 87536. HIV-2 RNA Quantitation is a send-out test performed by University of Washington Medical Center. Use the LAB4909 Laboratory Miscellaneous Order to order.

CPT Disclaimer

The Current Procedural Terminology (CPT) Codes published in the M Health Fairview Test Directory are based on American Medical Association (AMA) guidelines and are provided for informational purposes only. CPT codes are provided only as guidance to assist clients with billing. CPT coding is the responsibility of the billing party. M Health Fairview Laboratories does not assume responsibility for billing errors due to reliance on the CPT codes listed in this Test Directory. Charges may vary due to reflexing, susceptibilities, specimen source, patient age, methodology requirements, etc..

Patient Price Inquiries

Requester Contact Information
Patient and UMP/FV Care Team Fairview Consumer Line at 612-672-1048
MRL Outreach Client dept-mrl-business-assessment@fairview.org
Research research@fairview.org

CPT Codes

CPTQtyHC HospitalPR ClinicNote
87389130087389018738901HC HIV 1/2 ANTIGEN/ANTIBODY

Methodology

Electrochemiluminescence immunoassay

MRL Test Build

Test Name Component Required Description Type LOINC
LAB6327     HIV Antigen Antibody Combo Orderable  
  SRC_1001 Y Blood specimen source: Prompt  
  1230000088   HIV Antigen Antibody Combo Result 56888-1
 
Test Name Component Required Description Type LOINC
PLAB298     HIV 1/2 Supplemental Assay Performable  
  1230000066   HIV 1 Result Result 68961-2
  1230000067   HIV 2 Result Result 81641-3
  1230000068   HIV Interpretation Result 75666-8

Data Type / Multiple Choice Response

SRC_1001
Blood specimen source:
Arm, Left|Arm, Right|Hand, Left|Hand, Right|Blood, Capillary|Other|Peripheral Blood
Default: Peripheral Blood

Contact

For questions regarding the test code Interface Map, please contact DEPT-LAB-CLIENT-INTERFACE@fairview.org
Ordering

Special Instructions

It is recommended that HIV testing be discussed with the patient prior to collection of the specimen. Written patient consent for HIV testing is not required.

Reflex Testing

See Interpretive Data below.

Turnaround Time

Performed 24/7

Interpretive Data

The testing algorithm begins with the Roche Elecsys HIV Duo electrochemiluminescence immunoassay that simultaneously detects both antibody to human immunodeficiency virus (HIV-1 and HIV-2) and HIV-1 p24 antigen on the Roche Cobas instrument, performed at the East Bank campus. If the patient specimen is repeatedly reactive with the screening assay, it will be tested using the HIV Geenius (BioRad) HIV-1/2 immunoassay, which can differentiate between HIV-1 and HIV-2 antibodies. If the differentiation assay is also reactive, the presence of HIV-1 or HIV-2 antibodies is established. Specimens non-reactive with the HIV Geenius are reported as indeterminate and sent for HIV-1 RNA PCR testing performed by the Infectious Disease Diagnostic Laboratory. If HIV-2 is indeterminate, it is recommended to recollect and order testing for HIV-2 RNA Quantitation. If both HIV-1 and HIV-2 are positive, additional testing is required to rule out dual infection. The HIV Geenius CPT code is 86701 and 86702 and the Epic Code is LAB6328. The HIV Geenius will only be ordered by the Laboratory. The HIV-1 RNA Quantitation test code is LAB878 and the CPT code is 87536. HIV-2 RNA Quantitation is a send-out test performed by University of Washington Medical Center. Use the LAB4909 Laboratory Miscellaneous Order to order.

Associated Links

HIV Diagnostic Algorithm
Collection & Processing

Specimen Type

Blood

Collection Containers

10 mL Purple EDTA Tube
Alternate Containers: Green (lithium heparin, no gel), Green (lithium heparin, gel), Purple (EDTA), Red (no gel), Red or gold (gel)
 

Only for Blood and Body Fluid Exposure workups ordered via LAB704: 3 mL Purple (EDTA)

Collection Volume

9 mL

Minimum Collection Volume

5 mL

Only for Blood and Body Fluid Exposure workups ordered via LAB704: 3 mL

Note: this volume will not allow for any recommended reflex testing to occur

Specimen Preparation

Separate from cells within 24 hours of collection. 10 mL purple EDTA container recommended. If an alternate container is used the sample will not reflex to HIV 1 PCR testing.
Non-gel tubes: transfer serum/plasma to approved aliquot container.
Optimal testing volume: 2.5 mL serum/plasma; Minimum testing volume:1.55 mL

Unacceptable Conditions

Gross hemolysis or heat-inactivated specimens

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

After separation from cells:
Room temperature: 7 days
Refrigerated (preferred): 28 days
Frozen: 90 days (samples may be frozen up to 5 times)

Associated Links

HIV Diagnostic Algorithm
Containers

Containers

10 mL Purple EDTA Tube

Alternate Containers

Green (Lithium Heparin, No Gel)

Green (Lithium Heparin, Gel)

Purple (EDTA)

Red (No Gel)

Red or Gold (Gel)

Result Interpretation

Interpretive Data

The testing algorithm begins with the Roche Elecsys HIV Duo electrochemiluminescence immunoassay that simultaneously detects both antibody to human immunodeficiency virus (HIV-1 and HIV-2) and HIV-1 p24 antigen on the Roche Cobas instrument, performed at the East Bank campus. If the patient specimen is repeatedly reactive with the screening assay, it will be tested using the HIV Geenius (BioRad) HIV-1/2 immunoassay, which can differentiate between HIV-1 and HIV-2 antibodies. If the differentiation assay is also reactive, the presence of HIV-1 or HIV-2 antibodies is established. Specimens non-reactive with the HIV Geenius are reported as indeterminate and sent for HIV-1 RNA PCR testing performed by the Infectious Disease Diagnostic Laboratory. If HIV-2 is indeterminate, it is recommended to recollect and order testing for HIV-2 RNA Quantitation. If both HIV-1 and HIV-2 are positive, additional testing is required to rule out dual infection. The HIV Geenius CPT code is 86701 and 86702 and the Epic Code is LAB6328. The HIV Geenius will only be ordered by the Laboratory. The HIV-1 RNA Quantitation test code is LAB878 and the CPT code is 87536. HIV-2 RNA Quantitation is a send-out test performed by University of Washington Medical Center. Use the LAB4909 Laboratory Miscellaneous Order to order.
Administrative

CPT Disclaimer

The Current Procedural Terminology (CPT) Codes published in the M Health Fairview Test Directory are based on American Medical Association (AMA) guidelines and are provided for informational purposes only. CPT codes are provided only as guidance to assist clients with billing. CPT coding is the responsibility of the billing party. M Health Fairview Laboratories does not assume responsibility for billing errors due to reliance on the CPT codes listed in this Test Directory. Charges may vary due to reflexing, susceptibilities, specimen source, patient age, methodology requirements, etc..

Patient Price Inquiries

Requester Contact Information
Patient and UMP/FV Care Team Fairview Consumer Line at 612-672-1048
MRL Outreach Client dept-mrl-business-assessment@fairview.org
Research research@fairview.org

CPT Codes

CPTQtyHC HospitalPR ClinicNote
87389130087389018738901HC HIV 1/2 ANTIGEN/ANTIBODY

Methodology

Electrochemiluminescence immunoassay
Interface Mapping

MRL Test Build

Test Name Component Required Description Type LOINC
LAB6327     HIV Antigen Antibody Combo Orderable  
  SRC_1001 Y Blood specimen source: Prompt  
  1230000088   HIV Antigen Antibody Combo Result 56888-1
 
Test Name Component Required Description Type LOINC
PLAB298     HIV 1/2 Supplemental Assay Performable  
  1230000066   HIV 1 Result Result 68961-2
  1230000067   HIV 2 Result Result 81641-3
  1230000068   HIV Interpretation Result 75666-8

Data Type / Multiple Choice Response

SRC_1001
Blood specimen source:
Arm, Left|Arm, Right|Hand, Left|Hand, Right|Blood, Capillary|Other|Peripheral Blood
Default: Peripheral Blood

Contact

For questions regarding the test code Interface Map, please contact DEPT-LAB-CLIENT-INTERFACE@fairview.org
Private Details

Test Detail

Processing Key

Abbreviation
HIAGAB
Performing Lab
UMMC East Bank
Department
Chemistry
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