Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly labeltube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Room Temperature: 24 hours; Refrigerated (2-8°): 7 days; Frozen: >7 days at -20°
Ordering Recommendations
The ANA Ab IF QN test uses an indirect immunofluorescence technique to detect and quantitate a variety of autoantibodies in serum. The ANA test uses a HEp-2 substrate which consists of fixed smears of human epithelial cells. The ANA test is typically ordered as the initial screening test when there is a suspicion that the patient may have an autoimmune disease such as systemic lupus erythematosus.
Performed
Monday - Friday; Days
Methodology
Immunofluorescence
Reported
1-3 days
Performing Lab
Serology
Synonyms
4826
ANA
ANA Ab
Antinuclear Antibody (ANA) IF Quant
Antinuclear Antibody IF Quantitative
Reference Interval
<1:80 - No detectable anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA)
1:80 - Indeterminate for anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA).
Clinical-pathological correlation required; Approximately 13% of normal healthy individuals will test positive at the 1:80 dilution. A rheumatology consult may not be necessary.
>/= 1:160 Positive for anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA)
CPT Codes
86039
LOINC Codes
NAME
LOINC
Order
Antinuclear Antibody IF Quantitative
13111-0
Result
ANA Pattern
20399-2
ANA Second Pattern
14611-8
ANA Second Titer
29953-7
ANA Third Titer
29953-7
ANA Titer
5048-4
Cytoplasmic Interp
17352-6
Cytoplasmic Pattern
21419-7
Cytoplasmic Titer
32708-0
Test Code (Outreach Synonym)
4826
Catalog Code
692118
Collection
Collect
Blood, Gold
Preferred Draw Volume
2 mL
Minimum Draw Volume
1 mL
Other Acceptable Containers
Red
Pediatric Collection
2 microtainers
Collection Comments
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly labeltube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Room Temperature: 24 hours; Refrigerated (2-8°): 7 days; Frozen: >7 days at -20°
Ordering
Ordering Recommendations
The ANA Ab IF QN test uses an indirect immunofluorescence technique to detect and quantitate a variety of autoantibodies in serum. The ANA test uses a HEp-2 substrate which consists of fixed smears of human epithelial cells. The ANA test is typically ordered as the initial screening test when there is a suspicion that the patient may have an autoimmune disease such as systemic lupus erythematosus.
Performed
Monday - Friday; Days
Methodology
Immunofluorescence
Reported
1-3 days
Performing Lab
Serology
Synonyms
4826
ANA
ANA Ab
Antinuclear Antibody (ANA) IF Quant
Antinuclear Antibody IF Quantitative
Result Interpretation
Reference Interval
<1:80 - No detectable anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA)
1:80 - Indeterminate for anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA).
Clinical-pathological correlation required; Approximately 13% of normal healthy individuals will test positive at the 1:80 dilution. A rheumatology consult may not be necessary.
>/= 1:160 Positive for anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA)
