Whole blood or plasma on ice
Clotted sample
Sample collected in glass tube
Unspun specimen > 4 hours old
Underfilled tube
Gross hemolysis
Collection Comments
Full, 2.7 mL Blue, BD 3.2% Na Citrate plastic tube. Do not send whole blood or plasma to lab on ice. Double spin and freeze plasma within 4 hours of collection: Spin at 3000 rpm for 15 minutes. Place top 2/3 of plasma in aliquot tube and spin aliquot at 3000 rpm for 15 minutes. Take top 2/3 of 2nd spin and put in another aliquot tube. Mark tube 'plasma'. Freeze immediately. Send frozen platelet poor plasma. Send refrigerated RBC's in separate tube. DO NOT FREEZE red cells. Remember to properly label the aliquot tube with patient name, DOB, test and collect date.
Patients who have decreased ratios for APC Resistance have an increased risk for thromboembolic disease. Patients with recurrent thrombosis should be tested for resistance to activated protein C (APC Sensitivity) to determine its involvement in the disorder. The frequency of activated protein C resistance has been reported to occur at least tenfold more frequently than deficiencies of protein C, protein S, and antithrombin III combined. Familial activated protein C resistance can be confirmed by FactorV Leiden PCR.
Performed
Days; once/week
Methodology
Clottable
Reported
7 days
Performing Lab
Special Coag
Synonyms
567
Activated Protein C Resistance (APCR)
Reference Interval
>=2.4
CPT Codes
85307
LOINC Codes
NAME
LOINC
Order
Activated Protein C Resistance
13590-5
Result
APC Resistance
13590-5
Test Code (Outreach Synonym)
567
Catalog Code
667637
Collection
Collect
Blood, Light Blue
Preferred Draw Volume
2.7 mL
Minimum Draw Volume
Full tube
Unacceptable Conditions
Whole blood or plasma on ice
Clotted sample
Sample collected in glass tube
Unspun specimen > 4 hours old
Underfilled tube
Gross hemolysis
Collection Comments
Full, 2.7 mL Blue, BD 3.2% Na Citrate plastic tube. Do not send whole blood or plasma to lab on ice. Double spin and freeze plasma within 4 hours of collection: Spin at 3000 rpm for 15 minutes. Place top 2/3 of plasma in aliquot tube and spin aliquot at 3000 rpm for 15 minutes. Take top 2/3 of 2nd spin and put in another aliquot tube. Mark tube 'plasma'. Freeze immediately. Send frozen platelet poor plasma. Send refrigerated RBC's in separate tube. DO NOT FREEZE red cells. Remember to properly label the aliquot tube with patient name, DOB, test and collect date.
Patients who have decreased ratios for APC Resistance have an increased risk for thromboembolic disease. Patients with recurrent thrombosis should be tested for resistance to activated protein C (APC Sensitivity) to determine its involvement in the disorder. The frequency of activated protein C resistance has been reported to occur at least tenfold more frequently than deficiencies of protein C, protein S, and antithrombin III combined. Familial activated protein C resistance can be confirmed by FactorV Leiden PCR.
