Avoid repeated freeze/thaw cycles
Avoid hemolyzed or lipemic specimens
No plasma
Collection Comments
Fasting patient preferred. Process within 2 hours of collection. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Spun sample: Room Temperature: 2 days; Refrigerated: up to 8 days; Frozen: extended storage
Ordering Recommendations
Anti-streptolysin O (ASO) is an antibody directed against streptolysin O, a toxin produced by many strains of Streptococci. The test may be ordered when there is a suspicion of a recent streptococcal infection, however the ASO test is more frequently ordered when there are symptoms of a post-streptococcal disease such as rheumatic fever or glomerulonephritis.
Performed
Monday - Friday; Days
Methodology
Turbidimetry
Reported
1-3 days
Performing Lab
Special Chemistry
Synonyms
Antistreptolysin O (ASO) Antibodies
antistreptolysin
Anti Streptolysin O Ab
4703
Reference Chart
Age (years)
Lower Limit (IU/mL)
Upper Limit (IU/mL)
0 to <6
0
140
6 to <13
0
330
13 to <19
0
290
>19
0
200
CPT Codes
86060
LOINC Codes
NAME
LOINC
Order
Antistreptolysin
5370-2
Result
ASO
5370-2
Test Code (Outreach Synonym)
4703
Catalog Code
685369
Collection
Collect
Blood, Gold
Preferred Draw Volume
5 mL
Minimum Draw Volume
0.5 mL
Other Acceptable Containers
Red
Pediatric Collection
1 Full microtainer
Unacceptable Conditions
Avoid repeated freeze/thaw cycles
Avoid hemolyzed or lipemic specimens
No plasma
Collection Comments
Fasting patient preferred. Process within 2 hours of collection. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Spun sample: Room Temperature: 2 days; Refrigerated: up to 8 days; Frozen: extended storage
Ordering
Ordering Recommendations
Anti-streptolysin O (ASO) is an antibody directed against streptolysin O, a toxin produced by many strains of Streptococci. The test may be ordered when there is a suspicion of a recent streptococcal infection, however the ASO test is more frequently ordered when there are symptoms of a post-streptococcal disease such as rheumatic fever or glomerulonephritis.
