Gross lipemia. Hemolysis can interfere with assay.
Collection Comments
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date. If collecting in Red, spin, separate, transfer serum to aliquot tube and refrigerate.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
After separation from cells: Refrigerated: 7 days; Frozen: indefinitely
Ordering Recommendations
The Centromere B Ab EIA test uses an enzyme immunoassay to measure autoantibodies to the centromere protein B. Centromere protein B is a DNA-binding protein required for the formation of functioning centromeres. This test may be ordered when there is a suspicion of an autoimmune process, especially a form of scleroderma known as CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly and telangiectasia).
Performed
Monday - Saturday; Days
Methodology
Multiplex Flow Immunoassay
Reported
1-3 days
Performing Lab
Serology
Synonyms
998
Reference Interval
Ab Index < 1.0 - Negative; Ab Index >/= 1.0 - Positive
CPT Codes
86235
LOINC Codes
NAME
LOINC
Order
Centromere B Ab EIA
8068-9
Result
Centromere B Ab EIA
8068-9
Test Code (Outreach Synonym)
998
Catalog Code
703773585
Collection
Collect
Blood, Gold
Preferred Draw Volume
2 mL
Minimum Draw Volume
0.8 mL
Other Acceptable Containers
Red
Pediatric Collection
2 microtainers
Unacceptable Conditions
Gross lipemia. Hemolysis can interfere with assay.
Collection Comments
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date. If collecting in Red, spin, separate, transfer serum to aliquot tube and refrigerate.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
After separation from cells: Refrigerated: 7 days; Frozen: indefinitely
Ordering
Ordering Recommendations
The Centromere B Ab EIA test uses an enzyme immunoassay to measure autoantibodies to the centromere protein B. Centromere protein B is a DNA-binding protein required for the formation of functioning centromeres. This test may be ordered when there is a suspicion of an autoimmune process, especially a form of scleroderma known as CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly and telangiectasia).
Performed
Monday - Saturday; Days
Methodology
Multiplex Flow Immunoassay
Reported
1-3 days
Performing Lab
Serology
Synonyms
998
Result Interpretation
Reference Interval
Ab Index < 1.0 - Negative; Ab Index >/= 1.0 - Positive
