Hemolysis, icterus, lipemia, heat-treated or specimens other than serum
Collection Comments
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Room Temperature <=8 hours, 2-8°C < 48 hours, >= 48 hours freeze at -20°C
Ordering Recommendations
The ENA SCL70 Ab QN test is a quantitative enzyme immunoassay procedure used to assess the presence of antibodies to the extractable nuclear antigen SCL-70 (also known as topoisomerase I). The extractable nuclear antigen tests are frequently used to distinguish among autoimmune disorders. The ENA SCL-70 Ab QN test may be ordered as a follow up to a positive antinuclear antibody test (ANA) or when there is a suspicion of an autoimmune disease.
Performed
Monday, Wednesday, Friday; Days
Methodology
EIA
Reported
2-7 days
Performing Lab
Serology
Synonyms
10108
ENA SCL-70 AB
SCL-70 AB
Anti-topoisomerase
ENA SCL-70 AB EIA Quantitative
Scleroderma Ab
Reference Interval
Negative <20 Units
Weak Positive 20-39 Units
Moderate Positive 40-80 Units
Strong Positive >80 Units
CPT Codes
86235
LOINC Codes
NAME
LOINC
Order
Extractable Nuclear Antibodies (ENA Scleroderma-70), Quan
27416-7
Result
ENA SCL70 Ab QN
27416-7
ENA SCL70 Ab QN Interp
44083-4
Test Code (Outreach Synonym)
10108
Catalog Code
3769690335
Collection
Collect
Blood, Gold
Preferred Draw Volume
2 mL
Minimum Draw Volume
1 mL
Other Acceptable Containers
Red
Pediatric Collection
2 microtainers
Unacceptable Conditions
Hemolysis, icterus, lipemia, heat-treated or specimens other than serum
Collection Comments
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Room Temperature <=8 hours, 2-8°C < 48 hours, >= 48 hours freeze at -20°C
Ordering
Ordering Recommendations
The ENA SCL70 Ab QN test is a quantitative enzyme immunoassay procedure used to assess the presence of antibodies to the extractable nuclear antigen SCL-70 (also known as topoisomerase I). The extractable nuclear antigen tests are frequently used to distinguish among autoimmune disorders. The ENA SCL-70 Ab QN test may be ordered as a follow up to a positive antinuclear antibody test (ANA) or when there is a suspicion of an autoimmune disease.
Performed
Monday, Wednesday, Friday; Days
Methodology
EIA
Reported
2-7 days
Performing Lab
Serology
Synonyms
10108
ENA SCL-70 AB
SCL-70 AB
Anti-topoisomerase
ENA SCL-70 AB EIA Quantitative
Scleroderma Ab
Result Interpretation
Reference Interval
Negative <20 Units
Weak Positive 20-39 Units
Moderate Positive 40-80 Units
Strong Positive >80 Units
Additional Information
CPT Codes
86235
LOINC Codes
NAME
LOINC
Order
Extractable Nuclear Antibodies (ENA Scleroderma-70), Quan
