Gross lipemia. Hemolysis can interfere with assay.
Collection Comments
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date. If collecting in Red, spin, separate, transfer serum to aliquot tube and refrigerate.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
After separation from cells: Refrigerated: 7 days; Frozen: indefinitely
Ordering Recommendations
The ENA Smith/RNP Ab EIA test uses an enzyme immunoassay to measure antibodies to the extractable nuclear antigens ribonucleoprotein (RNP) and Smith (Sm). The extractable nuclear antigen tests are frequently used to distinguish among autoimmune disorders. The ENA Smith/RNP Ab test may be ordered as a follow up to a positive antinuclear antibody test (ANA) or when there is a suspicion of an autoimmune disease.
Performed
Monday - Saturday; Days
Methodology
Multiplex Flow Immunoassay
Reported
1-3 days
Performing Lab
Serology
Synonyms
4843
ENA RNP Ab
RNP ENA Ab
Ribonucleic Protein Ab
ENA Smith/RNP Ab GD QL
Reference Interval
Ab Index < 1.0 - Negative; Ab Index >/= 1.0 - Positive
CPT Codes
86235 (x2)
LOINC Codes
NAME
LOINC
Order
ENA Smith/RNP Ab EIA
27048-8
Result
ENA Smith Ab EIA
11090-8
ENA Smith Ab EIA Interp
31627-3
ENA Smith/RNP Ab EIA
27048-8
Test Code (Outreach Synonym)
4843
Catalog Code
703793400
Collection
Collect
Blood, Gold
Preferred Draw Volume
2 mL
Minimum Draw Volume
0.8 mL
Other Acceptable Containers
Red
Pediatric Collection
2 microtainers
Unacceptable Conditions
Gross lipemia. Hemolysis can interfere with assay.
Collection Comments
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date. If collecting in Red, spin, separate, transfer serum to aliquot tube and refrigerate.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
After separation from cells: Refrigerated: 7 days; Frozen: indefinitely
Ordering
Ordering Recommendations
The ENA Smith/RNP Ab EIA test uses an enzyme immunoassay to measure antibodies to the extractable nuclear antigens ribonucleoprotein (RNP) and Smith (Sm). The extractable nuclear antigen tests are frequently used to distinguish among autoimmune disorders. The ENA Smith/RNP Ab test may be ordered as a follow up to a positive antinuclear antibody test (ANA) or when there is a suspicion of an autoimmune disease.
Performed
Monday - Saturday; Days
Methodology
Multiplex Flow Immunoassay
Reported
1-3 days
Performing Lab
Serology
Synonyms
4843
ENA RNP Ab
RNP ENA Ab
Ribonucleic Protein Ab
ENA Smith/RNP Ab GD QL
Result Interpretation
Reference Interval
Ab Index < 1.0 - Negative; Ab Index >/= 1.0 - Positive
