Collect


Blood, Lavender

Preferred Draw Volume

6 mL

Minimum Draw Volume

2 mL

Other Acceptable Containers

Pearl, Gold

Unacceptable Conditions

Plasma not separated within 6 hours of collection
Whole Blood frozen
Specimen collected in heparin
Specimen contains less than 1 mL of plasma or serum
Specimens that have been tested on the automation line in Chemistry or Hematology

Collection Comments

No heparin. Do not collect in Orange tube.
Separate from cells within 6 hours of collection. Transfer 2 mL plasma to an ARUP standard transport tube.

Outreach clients - collect in pearl tube, spin and separate within 6 hours. Ship spun pearl top refrigerated. If collected in lavender or yellow, spin and separate within 6 hours of collection. Aliquot serum or plasma into plastic tube and refrigerate. Plasma is stable for 6 days refrigerated, if longer than 6 days, plasma must be frozen. Do not freeze pearl top tube—aliquot plasma and freeze. This quantitative test is an RNA assay capable of detecting less than 50 IU/mL consistently according to the NIH Consensus on Management of Hepatitis C.

Storage/Transport Temperature

Refrigerated serum/plasma

Stability (from collection to initiation)

Room Temperature: not recommended
Refrigerated: 6 days
Frozen: 84 days

Special Handling

Spin within 6 hrs, Refrigerate

Ordering Recommendations

Hepatitis C Virus (HCV) is considered to be the principle etiologic agent responsible for 90-95% of the cases of post-transfusion non-A and non-B hepatitis. This assay is used to monitor the HCV viral load which is a predictor as to how well the patientis responding to therapy. Since this assay can detect the amount of viral particles present in plasma or serum it is an indicator of an active HCV viremia. This test is not intended for the screening of blood or blood products for the presence of HCV. Primers and probes are specific for HCV genotypes 1-6.

Performed

Days; 5 days/week

Methodology

PCR-RT QN

Reported

2-3 days

Performing Lab

Molecular

Synonyms

  • 7463
  • Hepatitis C Virus (HCV) Quantitative PCR Test (Viral Load)
  • HCV Viral Load
  • Hepatitis C By Branched DNA QN
  • HCV RNA PCR
  • Hep C RNA/PCR

Reference Interval

Detected Range: 15 - 1.00 E8 IU/mL
Reference Interval: Not Detected

CPT Codes

87522

LOINC Codes

  NAME LOINC
Order Hepatitis C SerPl QN PCR 11011-4
     
Result Hep C SerPl QN PCR 11011-4
  Hep C SerPl QN Interp 11259-9
  Log10 Hep C RNA QN 38180-6

Test Code (Outreach Synonym)

7463

Catalog Code

795217
Collection

Collect


Blood, Lavender

Preferred Draw Volume

6 mL

Minimum Draw Volume

2 mL

Other Acceptable Containers

Pearl, Gold

Unacceptable Conditions

Plasma not separated within 6 hours of collection
Whole Blood frozen
Specimen collected in heparin
Specimen contains less than 1 mL of plasma or serum
Specimens that have been tested on the automation line in Chemistry or Hematology

Collection Comments

No heparin. Do not collect in Orange tube.
Separate from cells within 6 hours of collection. Transfer 2 mL plasma to an ARUP standard transport tube.

Outreach clients - collect in pearl tube, spin and separate within 6 hours. Ship spun pearl top refrigerated. If collected in lavender or yellow, spin and separate within 6 hours of collection. Aliquot serum or plasma into plastic tube and refrigerate. Plasma is stable for 6 days refrigerated, if longer than 6 days, plasma must be frozen. Do not freeze pearl top tube—aliquot plasma and freeze. This quantitative test is an RNA assay capable of detecting less than 50 IU/mL consistently according to the NIH Consensus on Management of Hepatitis C.

Storage/Transport Temperature

Refrigerated serum/plasma

Stability (from collection to initiation)

Room Temperature: not recommended
Refrigerated: 6 days
Frozen: 84 days

Special Handling

Spin within 6 hrs, Refrigerate
Ordering

Ordering Recommendations

Hepatitis C Virus (HCV) is considered to be the principle etiologic agent responsible for 90-95% of the cases of post-transfusion non-A and non-B hepatitis. This assay is used to monitor the HCV viral load which is a predictor as to how well the patientis responding to therapy. Since this assay can detect the amount of viral particles present in plasma or serum it is an indicator of an active HCV viremia. This test is not intended for the screening of blood or blood products for the presence of HCV. Primers and probes are specific for HCV genotypes 1-6.

Performed

Days; 5 days/week

Methodology

PCR-RT QN

Reported

2-3 days

Performing Lab

Molecular

Synonyms

  • 7463
  • Hepatitis C Virus (HCV) Quantitative PCR Test (Viral Load)
  • HCV Viral Load
  • Hepatitis C By Branched DNA QN
  • HCV RNA PCR
  • Hep C RNA/PCR
Result Interpretation

Reference Interval

Detected Range: 15 - 1.00 E8 IU/mL
Reference Interval: Not Detected
Additional Information

CPT Codes

87522

LOINC Codes

  NAME LOINC
Order Hepatitis C SerPl QN PCR 11011-4
     
Result Hep C SerPl QN PCR 11011-4
  Hep C SerPl QN Interp 11259-9
  Log10 Hep C RNA QN 38180-6

Test Code (Outreach Synonym)

7463

Catalog Code

795217