Samples should be centrifuged within 30 minutes of collection and same tube type used throughout entire procedure.
Invert tube 8-10 times to mix blood with anticoagulant. Centrifuge blood for 10 minutes at 1100-1300 g in a swing-bucket unit or 15 minutes for a fixed angle. Label with patient name, DOB, test and collect date. Glucose values may be falsely decreased when cells are not separated from plasma/serum within 30 minutes of collection.
Storage/Transport Temperature
Refrigerated serum/plasma
Stability (from collection to initiation)
Glucose in serum or plasma, free from hemolysis and bacterial contamination, and added preservatives, is stable for 8 hours when stored at room temperature (15-25°C) or 72 hours when stored refrigerated at 2-8°C.
Special Handling
Spin Immediately
Ordering Recommendations
Glucose Tolerance Testing 100 GM 3 HR (4 specimens). Contains: Gluc Fast GTT 100 gm, Gluc 1 HR GTT, Gluc 2 HR GTT, Gluc 3 HR GTT. The 3 hour 100 gram glucose tolerance test is ordered as a follow up test when the results of a pregnant patient's 1 hour 50 gram glucose tolerance test suggest the possibility of gestational diabetes.
Performed
Monday - Sunday; Days, Evenings, Nights
Methodology
Hexokinase-UV/NAD
Reported
2-4 hours
Performing Lab
Chemistry
Synonyms
6254
OGTT
Glucose Tolerance Test, 100 gm, 3 hr
Reference Chart
CPT Codes
82951 (x3), 82952
Test Code (Outreach Synonym)
6254
Catalog Code
697833
Collection
Collect
Blood, Lt Green
Preferred Draw Volume
4.5 mL
Minimum Draw Volume
1 mL Whole Blood
Other Acceptable Containers
Gold
Pediatric Collection
1 Lt. Green Microtainer
Collection Comments
Samples should be centrifuged within 30 minutes of collection and same tube type used throughout entire procedure.
Invert tube 8-10 times to mix blood with anticoagulant. Centrifuge blood for 10 minutes at 1100-1300 g in a swing-bucket unit or 15 minutes for a fixed angle. Label with patient name, DOB, test and collect date. Glucose values may be falsely decreased when cells are not separated from plasma/serum within 30 minutes of collection.
Storage/Transport Temperature
Refrigerated serum/plasma
Stability (from collection to initiation)
Glucose in serum or plasma, free from hemolysis and bacterial contamination, and added preservatives, is stable for 8 hours when stored at room temperature (15-25°C) or 72 hours when stored refrigerated at 2-8°C.
Special Handling
Spin Immediately
Ordering
Ordering Recommendations
Glucose Tolerance Testing 100 GM 3 HR (4 specimens). Contains: Gluc Fast GTT 100 gm, Gluc 1 HR GTT, Gluc 2 HR GTT, Gluc 3 HR GTT. The 3 hour 100 gram glucose tolerance test is ordered as a follow up test when the results of a pregnant patient's 1 hour 50 gram glucose tolerance test suggest the possibility of gestational diabetes.
