For each cortisol sample: Samples thawed more than once
Collection Comments
To arrange for Outpatients and Outreach: fax the order or send message through Cerner Message Center (in PowerChart) to UH IUSCC Pre Cert Team with ICD Code, how often patient is getting it, and J code of the medicine. Place contact number in message or fax order to 317-948-6849, Attention Belinda at the Simon Cancer Center Infusion to have testing done. Belinda will call patient to schedule for testing.
Draw in AM for best results. Draw Pre, 30 min Post, and 60 Min Post Cortisol. Label each tube with the collection date and time. Invert tube5-6 times to mix blood. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle. Remember to label aliquot with patient name, DOB, test and collect date. Ship spun Orange, Lt Green or Gold tube refrigerated.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
For each cortisol sample:
Spun samples: Room Temperature: 8 hours; Refrigerated: 48 hours; Frozen: > 48 hours
Ordering Recommendations
The ACTH or cosyntropin stimulation test, is ordered when there is a suspicion of primary or secondary adrenal insufficiency. The test requires a baseline blood sample followed by administration of synthetic ACTH. Blood is then sampled at 30 and 60 minutes for additional measurements.
Performed
Monday - Friday; Days, Evenings
Methodology
Chemiluminescence
Performing Lab
Chemistry
Other Performing Labs
Ball
Synonyms
7513
Adrenocorticotrophic Hormone (ACTH) 1 hr Stimulation Ser QN
Cosyntropin ACTH Stimulation Test
Reference Chart
ACTH 1 HR Stim Ser QN
Reference Range
*Cortisol Pre
8-25 mcg/dL
*Cortisol Ser 30 min Post
At 30 min, cortisol should be at least 7 mcg/dL higher than baseline control
*Cortisol Ser 60 min Post
undefined mcg/dL
CPT Codes
82533 (x3)
Test Code (Outreach Synonym)
7513
Catalog Code
698926
Collection
Collect
Blood, Orange
Preferred Draw Volume
3 mL
Minimum Draw Volume
3 mL
Other Acceptable Containers
Gold, Lt Green
Unacceptable Conditions
For each cortisol sample: Samples thawed more than once
Collection Comments
To arrange for Outpatients and Outreach: fax the order or send message through Cerner Message Center (in PowerChart) to UH IUSCC Pre Cert Team with ICD Code, how often patient is getting it, and J code of the medicine. Place contact number in message or fax order to 317-948-6849, Attention Belinda at the Simon Cancer Center Infusion to have testing done. Belinda will call patient to schedule for testing.
Draw in AM for best results. Draw Pre, 30 min Post, and 60 Min Post Cortisol. Label each tube with the collection date and time. Invert tube5-6 times to mix blood. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle. Remember to label aliquot with patient name, DOB, test and collect date. Ship spun Orange, Lt Green or Gold tube refrigerated.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
For each cortisol sample:
Spun samples: Room Temperature: 8 hours; Refrigerated: 48 hours; Frozen: > 48 hours
Ordering
Ordering Recommendations
The ACTH or cosyntropin stimulation test, is ordered when there is a suspicion of primary or secondary adrenal insufficiency. The test requires a baseline blood sample followed by administration of synthetic ACTH. Blood is then sampled at 30 and 60 minutes for additional measurements.
Performed
Monday - Friday; Days, Evenings
Methodology
Chemiluminescence
Performing Lab
Chemistry
Other Performing Labs
Ball
Synonyms
7513
Adrenocorticotrophic Hormone (ACTH) 1 hr Stimulation Ser QN
Cosyntropin ACTH Stimulation Test
Result Interpretation
Reference Chart
ACTH 1 HR Stim Ser QN
Reference Range
*Cortisol Pre
8-25 mcg/dL
*Cortisol Ser 30 min Post
At 30 min, cortisol should be at least 7 mcg/dL higher than baseline control
