Bone Marrow or whole blood in lavender-top (EDTA) vacutainer.
>2 mL bone marrow aspirate or >5 mL of blood.
Additional Requirements
Pre-transplant patient and donor samples: 1) Archived DNA: DNA will be available in the CUMC Immunogenetics and Cellular Immunology from prior HLA genotyping testing during the pre-transplant evaluation. 2) Archived peripheral blood leukocytes (PBL): If DNA of pre-transplant patient and donor is not available, previous frozen PBL can be obtained from the CUMC Immunogenetics and Cellular Immunology from prior HLA genotyping testing during the pre-transplant evaluation.
Unacceptable Conditions
Broken tube, mismatched or missing labels, coagulated, blood >48 hours old, bone marrow >72 hours old, insufficient cellularity, improper anticoagulation, viability <50%, no history provided
Storage/Transport Temperature
Room temperature. Do not freeze.
Stability (from collection to initiation)
48 hours at room temperature (blood), 72 hours at room temperature (bone marrow).
Performed
Monday through Friday from 9 A.M. to 5 P.M.
Reported
4-6 business days
Panel Components
% donor cells.
Lab info
NewYork Presbyterian Hospital - Columbia University Irving Medical Center
Section
Immunogenetics and Cellular Immunology
Methodology
Flow cytometry, cell sorting, PCR, and gene fragment analysis
Reference Interval
No Reference Range
Can this test be added to a previous specimen?
No
CPT Codes
81265 and 81266 (pre-transplant); 81267 (post-transplant), 81268 (each additional lineage)
Test Information
Patient Preparation
Needle aspirate or routine venipuncture.
Collect
Bone Marrow or whole blood in lavender-top (EDTA) vacutainer.
>2 mL bone marrow aspirate or >5 mL of blood.
Additional Requirements
Pre-transplant patient and donor samples: 1) Archived DNA: DNA will be available in the CUMC Immunogenetics and Cellular Immunology from prior HLA genotyping testing during the pre-transplant evaluation. 2) Archived peripheral blood leukocytes (PBL): If DNA of pre-transplant patient and donor is not available, previous frozen PBL can be obtained from the CUMC Immunogenetics and Cellular Immunology from prior HLA genotyping testing during the pre-transplant evaluation.
Unacceptable Conditions
Broken tube, mismatched or missing labels, coagulated, blood >48 hours old, bone marrow >72 hours old, insufficient cellularity, improper anticoagulation, viability <50%, no history provided
Storage/Transport Temperature
Room temperature. Do not freeze.
Stability (from collection to initiation)
48 hours at room temperature (blood), 72 hours at room temperature (bone marrow).
Performed
Monday through Friday from 9 A.M. to 5 P.M.
Reported
4-6 business days
Panel Components
% donor cells.
Lab info
NewYork Presbyterian Hospital - Columbia University Irving Medical Center
Section
Immunogenetics and Cellular Immunology
Methodology
Flow cytometry, cell sorting, PCR, and gene fragment analysis
Reference Interval
No Reference Range
Can this test be added to a previous specimen?
No
CPT Codes
81265 and 81266 (pre-transplant); 81267 (post-transplant), 81268 (each additional lineage)
