Collect

Heme samples : one 4.5 mL blood in EDTA (lav top), or one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells). Yellow top (ACD) Vacutainers are also acceptable.
Fresh tissue: min 0.1 mL
Frozen tissue: min 100mg or 0.5 cc / 0.5 cm3
Body Fluids: >200K cells
Formalin-Fixed, paraffin-embedded tissue (FFPE). Please include the surgical pathology report with an estimate of tumor cellularity in the marked region.

Microtainer: 3 - 5 µM slices or more than 100,000 cells.
Slides: Provide 10 unstained slides with 10 µM slices, along with an associated H&E slide where tumor cells are clearly marked

Storage/Transport Temperature

Heme samples: 20-25 °C for several hours acceptable
Fresh tissue should be sent in saline-soaked gauze, a tube with saline, or tissue culture medium. 
Frozen samples should be sent on dry ice.
FFPE samples may be transported at ambient temperature; however, they should be protected from exposure to excessive heat during transit to maintain specimen integrity.

Unacceptable Conditions

Sample is rejected if specimen identity is in doubt.

Remarks

This test is lab-ordered only.

Stability (from collection to initiation)

Ambient: 72 hours: Refrigerated: 1 week
Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium.  Frozen samples should be sent on dry ice.
FFPE samples: Ambient: Up to 60 days recommended.  FFPE samples stored ambient beyond 60 days are not refused.  Acceptability is determined by testing performance.

Lab Section

Molecular Diagnositcs

Methodology

Targeted Next Generation Sequencing (NGS)

Performed

2 - 4x per week

Reported

7 - 10 working days

Synonyms

  • Heme Malignancy NGS
  • ABL1
  • ANKRD26
  • ASXL1
  • BCOR
  • BCOR1
  • BRAF
  • CALR
  • CBL
  • CEBPA
  • CSF3R
  • DDX41
  • DNMT3A
  • ETV6
  • EZH2
  • FLT3
  • GATA1
  • GATA2
  • HRAS
  • IDH1
  • IDH2
  • IKZF1
  • JAK2
  • KIT
  • KRAS
  • MPL
  • MYD88
  • NF1
  • NPM1
  • NRAS
  • PHF6
  • PPM1D
  • PTPN11
  • RAD21
  • RB1
  • RUNX1
  • SETBP1
  • SF3B1
  • SH2B3
  • SMC1A
  • SMC3
  • SRSF2
  • STAG2
  • STAT3
  • TET2
  • TP53
  • U2AF1
  • UBA1
  • WT1
  • ZRSR2
  • OMAP

Performing Laboratory Website (click below)

Notes

MCMP: ABL1, ANKRD26, ASXL1, BCOR, BCORL1, BRAF, CALR, CBL, CEBPA, CSF3R, DDX41, DNMT3A, ETV6, EZH2, FLT3, GATA1, GATA2, HRAS, IDH1, IDH2, IKZF1, JAK2, KIT, KRAS, MPL, MYD88, NF1, NPM1, NRAS, PHF6, PPM1D, PTPN11, RAD21, RB1, RUNX1, SETBP1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, TET2, TP53, U2AF1, UBA1, WT1, ZRSR2

Interpretive Data

Resulted as part of Pathology report. 

CPT Codes

81450

LOINC Mapping

73977-1

CPT Codes

81450

LOINC Mapping

73977-1
Specimen Requirements

Collect

Heme samples : one 4.5 mL blood in EDTA (lav top), or one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells). Yellow top (ACD) Vacutainers are also acceptable.
Fresh tissue: min 0.1 mL
Frozen tissue: min 100mg or 0.5 cc / 0.5 cm3
Body Fluids: >200K cells
Formalin-Fixed, paraffin-embedded tissue (FFPE). Please include the surgical pathology report with an estimate of tumor cellularity in the marked region.

Microtainer: 3 - 5 µM slices or more than 100,000 cells.
Slides: Provide 10 unstained slides with 10 µM slices, along with an associated H&E slide where tumor cells are clearly marked

Storage/Transport Temperature

Heme samples: 20-25 °C for several hours acceptable
Fresh tissue should be sent in saline-soaked gauze, a tube with saline, or tissue culture medium. 
Frozen samples should be sent on dry ice.
FFPE samples may be transported at ambient temperature; however, they should be protected from exposure to excessive heat during transit to maintain specimen integrity.

Unacceptable Conditions

Sample is rejected if specimen identity is in doubt.

Remarks

This test is lab-ordered only.

Stability (from collection to initiation)

Ambient: 72 hours: Refrigerated: 1 week
Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium.  Frozen samples should be sent on dry ice.
FFPE samples: Ambient: Up to 60 days recommended.  FFPE samples stored ambient beyond 60 days are not refused.  Acceptability is determined by testing performance.
Testing

Lab Section

Molecular Diagnositcs

Methodology

Targeted Next Generation Sequencing (NGS)

Performed

2 - 4x per week

Reported

7 - 10 working days

Synonyms

  • Heme Malignancy NGS
  • ABL1
  • ANKRD26
  • ASXL1
  • BCOR
  • BCOR1
  • BRAF
  • CALR
  • CBL
  • CEBPA
  • CSF3R
  • DDX41
  • DNMT3A
  • ETV6
  • EZH2
  • FLT3
  • GATA1
  • GATA2
  • HRAS
  • IDH1
  • IDH2
  • IKZF1
  • JAK2
  • KIT
  • KRAS
  • MPL
  • MYD88
  • NF1
  • NPM1
  • NRAS
  • PHF6
  • PPM1D
  • PTPN11
  • RAD21
  • RB1
  • RUNX1
  • SETBP1
  • SF3B1
  • SH2B3
  • SMC1A
  • SMC3
  • SRSF2
  • STAG2
  • STAT3
  • TET2
  • TP53
  • U2AF1
  • UBA1
  • WT1
  • ZRSR2
  • OMAP

Performing Laboratory Website (click below)

Notes

MCMP: ABL1, ANKRD26, ASXL1, BCOR, BCORL1, BRAF, CALR, CBL, CEBPA, CSF3R, DDX41, DNMT3A, ETV6, EZH2, FLT3, GATA1, GATA2, HRAS, IDH1, IDH2, IKZF1, JAK2, KIT, KRAS, MPL, MYD88, NF1, NPM1, NRAS, PHF6, PPM1D, PTPN11, RAD21, RB1, RUNX1, SETBP1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, TET2, TP53, U2AF1, UBA1, WT1, ZRSR2
Result Interpretation

Interpretive Data

Resulted as part of Pathology report. 
Coding

CPT Codes

81450

LOINC Mapping

73977-1
URM Labs Internal
Test Build

CPT Codes

81450

LOINC Mapping

73977-1