Whole blood or bone marrow in a lavender top (EDTA) Vacutainer, or purified cell populations from those tissues. Yellow top (ACD) Vacutainers are also acceptable
Storage/Transport Temperature
5 mL whole blood or 3 mL bone marrow at 2-8°C. (Min: 1 mL)
Remarks
Analysis of a donor specimen and a pre-transplant recipient specimen is required to select informative markers prior to post-transplant Chimerism Analysis.
Stability (from collection to initiation)
Ambient: several hours; Refrigerated: days; Frozen: Unacceptable
Lab Section
Molecular Diagnostics
Methodology
Polymerase Chain Reaction/ Fragment Analysis by capillary electrophoresis
Performed
As needed
Reported
Post-transplant: 5 working days; Pre-transplant: 14 days from receipt of both samples
Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu
Specimen Requirements
Collect
Whole blood or bone marrow in a lavender top (EDTA) Vacutainer, or purified cell populations from those tissues. Yellow top (ACD) Vacutainers are also acceptable
Storage/Transport Temperature
5 mL whole blood or 3 mL bone marrow at 2-8°C. (Min: 1 mL)
Remarks
Analysis of a donor specimen and a pre-transplant recipient specimen is required to select informative markers prior to post-transplant Chimerism Analysis.
Stability (from collection to initiation)
Ambient: several hours; Refrigerated: days; Frozen: Unacceptable
Testing
Lab Section
Molecular Diagnostics
Methodology
Polymerase Chain Reaction/ Fragment Analysis by capillary electrophoresis
Performed
As needed
Reported
Post-transplant: 5 working days; Pre-transplant: 14 days from receipt of both samples
