Provides a calculated value for free testosterone concentration using total testosterone measured by mass spectrometry. May be used to evaluate hyperandrogenism in children and cisgender females, monitor testosterone-suppressing hormone therapies (eg, antiandrogens or estrogens), evaluate testosterone status in individuals with protein-binding abnormalities, or evaluate hypogonadism in cisgender males.
To compare this test to other testosterone tests, refer to the ARUP Testosterone Tests Comparison table.
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay/Calculation
Reported
1-5 days
Synonyms
81059
LAB8162
Notes
Total testosterone and SHBG are measured and free testosterone is estimated from these measurements.
Reference Interval
Components
Reference Interval
Testosterone, Free by Mass Spec
Age
Male (pg/mL)
Female (pg/mL)
1-6 years
Less than 0.6
Less than 0.6
7-9 years
0.1-0.9
0.6-1.8
10-11
0.1-6.3
0.1-3.5
12-13
0.5-98.0
0.9-6.8
14-15
3-138.0
1.2-7.5
16-17
38.0-173.0
1.2-9.9
18 years and older
47-244
Not Applicable
18-30
Not Applicable
0.8-7.4
31-40
Not Applicable
1.3-9.2
41-51
Not Applicable
1.1-5.8
Postmenopausal
Not Applicable
0.6-3.8
Tanner Stage I
Less than or equal to 3.7
Less than 2.2
Tanner Stage II
0.3-21
0.4-4.5
Tanner Stage III
1.0-98.0
1.3-7.5
Tanner Stage IV
35.0-169.0
1.1-15.5
Tanner Stage V
41.0-239.0
0.8-9.2
Interpretive Data
Free testosterone concentration is calculated using total testosterone (measured by mass spectrometry) and the binding constant of testosterone and sex hormone-binding globulin (SHBG).
For individuals on testosterone-suppressing hormone therapies (e.g., antiandrogens or estrogens), refer to cisgender female reference intervals. For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0081059.
CPT Codes
84402
LOINC
13967-5
49042-5
Performing Lab
ARUP Laboratories
Collection
Department
Sendouts:FTESF ARUP 0081059 5/22/2014
Patient Preparation
Collect between 6-10 a.m.
Collect
Serum separator tube or green (sodium or lithium heparin).
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP standard transport tube. (Min: 0.8 mL)
Storage/Transport Temperature
Refrigerated.
Unacceptable Conditions
EDTA plasma.
Stability (from collection to initiation)
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
Provides a calculated value for free testosterone concentration using total testosterone measured by mass spectrometry. May be used to evaluate hyperandrogenism in children and cisgender females, monitor testosterone-suppressing hormone therapies (eg, antiandrogens or estrogens), evaluate testosterone status in individuals with protein-binding abnormalities, or evaluate hypogonadism in cisgender males.
To compare this test to other testosterone tests, refer to the ARUP Testosterone Tests Comparison table.
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay/Calculation
Reported
1-5 days
Synonyms
81059
LAB8162
Notes
Total testosterone and SHBG are measured and free testosterone is estimated from these measurements.
Result Interpretation
Reference Interval
Components
Reference Interval
Testosterone, Free by Mass Spec
Age
Male (pg/mL)
Female (pg/mL)
1-6 years
Less than 0.6
Less than 0.6
7-9 years
0.1-0.9
0.6-1.8
10-11
0.1-6.3
0.1-3.5
12-13
0.5-98.0
0.9-6.8
14-15
3-138.0
1.2-7.5
16-17
38.0-173.0
1.2-9.9
18 years and older
47-244
Not Applicable
18-30
Not Applicable
0.8-7.4
31-40
Not Applicable
1.3-9.2
41-51
Not Applicable
1.1-5.8
Postmenopausal
Not Applicable
0.6-3.8
Tanner Stage I
Less than or equal to 3.7
Less than 2.2
Tanner Stage II
0.3-21
0.4-4.5
Tanner Stage III
1.0-98.0
1.3-7.5
Tanner Stage IV
35.0-169.0
1.1-15.5
Tanner Stage V
41.0-239.0
0.8-9.2
Interpretive Data
Free testosterone concentration is calculated using total testosterone (measured by mass spectrometry) and the binding constant of testosterone and sex hormone-binding globulin (SHBG).
For individuals on testosterone-suppressing hormone therapies (e.g., antiandrogens or estrogens), refer to cisgender female reference intervals. For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0081059.
Laboratories.