EPIC Code

RESP PCR UCH

Orders must be placed using the Epic Wizard, “COVID-19/FLU/RESP VIRUS PANEL WIZARD.” Restrictions may apply.

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Molecular Diagnostics

Collect

Specimen Source 
  • Bronchoalveolar lavage (BAL) or bronchial wash
  • Tracheal aspirate

Minimum volume: >0.3 mL

Specimen Preparation

BAL, bronchial wash, or tracheal aspirate: Transfer 1 mL (Min: >0.3 mL) of sample to a sterile, non-additive container. Do not transfer fluid to media, including viral transport media.

Nasal swab: Flocked swab placed in M6/VTM/UTM/sterile PBS.

Unacceptable Conditions

  • Sources other than those listed.
  • Respiratory fluid (e.g., BAL) submitted in media.
  • ESwab®.
  • Wooden-shafted, calcium alginate, or cotton swabs.
  • Sample not properly identified, incorrect container, insufficient volume, or past stability.

Storage/Transport Temperature

Internal: Ambient
Offsite: Refrigerated

Performed

Daily

Stability (from collection to initiation)

Room Temp: 4 hours
Refrigerated: 3 days
Frozen: 31 days

Remarks

​​​​​​Specimen source must be identified on order or requisition.

This test includes qualitative testing for the following:
Adenovirus, Coronavirus 229E, Coronavirus HKU1, Coronavirus NL63, Coronavirus OC43, SARS-COV-2, Human Metapneumovirus, Rhinovirus/Enterovirus, Influenza A, Influenza A H1 2009, Influenza A H1, Influenza A H3, Influenza B, Parainfluenza 1,2,3, and 4, Respiratory Syncytial Virus, Bordetella pararapertussis, Bordetella pertussisChlamydia pneumoniae, Mycoplasma pneumoniae.

Performed

Daily

Methodology

Multiplex Film Array

Reported

Same day

Synonyms

  • RESP PCR UCH
  • RESPPCR
  • RVP PCR
  • Respiratory Viral Panel by PCR, Alt Source
  • Respiratory PCR Panel
  • Respiratory Pathogen Panel PCR Alternative Sources
  • LAB1989 LAB USE ONLY

EPIC Code

RESP PCR UCH

Orders must be placed using the Epic Wizard, “COVID-19/FLU/RESP VIRUS PANEL WIZARD.” Restrictions may apply.

Reference Interval

BioFire® FilmArray® RP2.1
ADENOVIRUS Not detected
CORONAVIRUS 229E Not detected
CORONAVIRUS HKU1 Not detected
CORONAVIRUS NL63 Not detected
CORONAVIRUS OC43 Not detected
SARS-COV-2 Not detected
HUMAN METAPNEUMOVIRUS Not detected
RHINOVIRUS/ENTEROVIRUS Not detected
INFLUENZA A Not detected
INFLUENZA A H1 2009 Not detected
INFLUENZA A H1 Not detected
INFLUENZA A H3 Not detected
INFLUENZA A (NO SUBTYPE DETECTED) Not detected
INFLUENZA B Not detected
PARAINFLUENZA 1 Not detected
PARAINFLUENZA 2 Not detected
PARAINFLUENZA 3 Not detected
PARAINFLUENZA 4 Not detected
RESPIRATORY SYNCYTIAL VIRUS Not detected
B PARAPERTUSSIS Not detected
B PERTUSSIS Not detected
C PNEUMONIAE Not detected
M PNEUMONIAE Not detected

Interpretive Data

Interpretation:
Not detected = Negative, the virus or bacteria indicated was not detected.
Detected = Positive, the virus or bacteria indicated was detected.

Methodology:
This test employs multiplex PCR amplification of viral and bacterial-specific conserved genetic targets. A positive result for this assay should be coupled with clinical indicators for diagnosis.  A "Not detected" result for this assay does not exclude involvement of the tested viruses or bacteria in a disease process. This test has been approved by the U.S. Food and Drug Administration for nasopharyngeal swab testing.  This laboratory is certified under the Clinical Laboratory Improvement Amendment of 1988 ("CLIA") as qualified to perform high complexity clinical testing.

CPT Codes

87633
Collection

EPIC Code

RESP PCR UCH

Orders must be placed using the Epic Wizard, “COVID-19/FLU/RESP VIRUS PANEL WIZARD.” Restrictions may apply.

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Molecular Diagnostics

Collect

Specimen Source 
  • Bronchoalveolar lavage (BAL) or bronchial wash
  • Tracheal aspirate

Minimum volume: >0.3 mL

Specimen Preparation

BAL, bronchial wash, or tracheal aspirate: Transfer 1 mL (Min: >0.3 mL) of sample to a sterile, non-additive container. Do not transfer fluid to media, including viral transport media.

Nasal swab: Flocked swab placed in M6/VTM/UTM/sterile PBS.

Unacceptable Conditions

  • Sources other than those listed.
  • Respiratory fluid (e.g., BAL) submitted in media.
  • ESwab®.
  • Wooden-shafted, calcium alginate, or cotton swabs.
  • Sample not properly identified, incorrect container, insufficient volume, or past stability.

Storage/Transport Temperature

Internal: Ambient
Offsite: Refrigerated

Performed

Daily

Stability (from collection to initiation)

Room Temp: 4 hours
Refrigerated: 3 days
Frozen: 31 days

Remarks

​​​​​​Specimen source must be identified on order or requisition.

This test includes qualitative testing for the following:
Adenovirus, Coronavirus 229E, Coronavirus HKU1, Coronavirus NL63, Coronavirus OC43, SARS-COV-2, Human Metapneumovirus, Rhinovirus/Enterovirus, Influenza A, Influenza A H1 2009, Influenza A H1, Influenza A H3, Influenza B, Parainfluenza 1,2,3, and 4, Respiratory Syncytial Virus, Bordetella pararapertussis, Bordetella pertussisChlamydia pneumoniae, Mycoplasma pneumoniae.
Ordering

Performed

Daily

Methodology

Multiplex Film Array

Reported

Same day

Synonyms

  • RESP PCR UCH
  • RESPPCR
  • RVP PCR
  • Respiratory Viral Panel by PCR, Alt Source
  • Respiratory PCR Panel
  • Respiratory Pathogen Panel PCR Alternative Sources
  • LAB1989 LAB USE ONLY

EPIC Code

RESP PCR UCH

Orders must be placed using the Epic Wizard, “COVID-19/FLU/RESP VIRUS PANEL WIZARD.” Restrictions may apply.
Result Interpretation

Reference Interval

BioFire® FilmArray® RP2.1
ADENOVIRUS Not detected
CORONAVIRUS 229E Not detected
CORONAVIRUS HKU1 Not detected
CORONAVIRUS NL63 Not detected
CORONAVIRUS OC43 Not detected
SARS-COV-2 Not detected
HUMAN METAPNEUMOVIRUS Not detected
RHINOVIRUS/ENTEROVIRUS Not detected
INFLUENZA A Not detected
INFLUENZA A H1 2009 Not detected
INFLUENZA A H1 Not detected
INFLUENZA A H3 Not detected
INFLUENZA A (NO SUBTYPE DETECTED) Not detected
INFLUENZA B Not detected
PARAINFLUENZA 1 Not detected
PARAINFLUENZA 2 Not detected
PARAINFLUENZA 3 Not detected
PARAINFLUENZA 4 Not detected
RESPIRATORY SYNCYTIAL VIRUS Not detected
B PARAPERTUSSIS Not detected
B PERTUSSIS Not detected
C PNEUMONIAE Not detected
M PNEUMONIAE Not detected

Interpretive Data

Interpretation:
Not detected = Negative, the virus or bacteria indicated was not detected.
Detected = Positive, the virus or bacteria indicated was detected.

Methodology:
This test employs multiplex PCR amplification of viral and bacterial-specific conserved genetic targets. A positive result for this assay should be coupled with clinical indicators for diagnosis.  A "Not detected" result for this assay does not exclude involvement of the tested viruses or bacteria in a disease process. This test has been approved by the U.S. Food and Drug Administration for nasopharyngeal swab testing.  This laboratory is certified under the Clinical Laboratory Improvement Amendment of 1988 ("CLIA") as qualified to perform high complexity clinical testing.
Administrative

CPT Codes

87633