Specimen must be centrifuged and plasma removed to another tube within two hours of collection. Centrifuge at 3,400 RPM's for 10 minutes. Follow directions in the centrifuge manual to set the centrifuge appropriately. Transfer the plasma using a disposable pipette or pouring to another labeled tube (pour off tube). Rubber band the original tube (parent tube) with the labeled pour off tube prior to sending the specimen to CLS/Immunology Lab. Separated plasma must be stored at -80C if Clinical Immunology Lab is closed.
Unacceptable Conditions
Severely hemolyzed and/ or grossly lipemic samples. Any specimen that has NOT been centrifuged and split within 2 hours of collection.
Storage/Transport Temperature
Transport at room temperature.
Store separated plasma at -80C.
Stability (from collection to initiation)
Sample is stable in PST tube for 2 hours. Centrifuge and remove plasma to another tube (pour off tube) within two hours of collection.
Remarks
Panel includes:
Interferon gamma (IFN-g)
Interleukin 1 beta (IL-1b)
Interleukin 2 (IL-2)
Interleukin 4 (IL-4)
Interleukin 6 (IL-6)
Interleukin 8 (IL-8)
Interleukin 10 (IL-10)
Interleukin 12p70 (IL-12p70)
Interleukin 13 (IL-13)
Tumor Necrosis Factor alpha (TNF-a)
Volume Required
3.0 ml
Minimum Required
1.0 ml
Phlebotomy Draw
Yes
Performing Lab
Immunology Lab
Performed
Minimum of 2 days/week.
Methodology
Electrochemiluminescence
Reported
3 days
Synonyms
Cytokine
Cytokines
Interferon gamma
IFN-g
Interleukin 1 beta
IL-1b
Interleukin 2
IL-2
Interleukin 4
IL-4
Interleukin 6
IL-6
Interleukin 8
IL-8
Interleukin 10
IL-10
Interleukin 12p70
IL-12p70
Interleukin 13
IL-13
Tumor Necrosis Factor alpha
TNF-a
LIS Mnemonic
CYTK
Available STAT
Yes - requires Medical Director approval & DIRT consult
Interpretive Data
Any deviations from specimen processing instructions may cause unexpected results in all cytokines. Specifically for Interleukin 8 (IL-8), significantly falsely increased results may be observed.
This test was developed and its performance characteristics determined by the Clinical Immunology Laboratory of The Children's Hospital of Philadelphia. It has not been cleared or approved by the US Food and Drug Administration (FDA). This laboratory is regulated under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.
Reference Ranges
Analyte
Normal range (pg/ml)
IFN gamma
< 6.5
IL-1 beta
< 2.5
IL-2
< 6.5
IL-4
< 1.5
IL-6
< 3.5
IL-8
< 10.0
IL-10
< 2.0
IL-12 p70
< 2.5
IL-13
< 2.5
TNF alpha
<3.5
CPT Codes
83520 x9, 83529 (IL-6)
Interpretive Data
Any deviations from specimen processing instructions may cause unexpected results in all cytokines. Specifically for Interleukin 8 (IL-8), significantly falsely increased results may be observed.
This test was developed and its performance characteristics determined by the Clinical Immunology Laboratory of The Children's Hospital of Philadelphia. It has not been cleared or approved by the US Food and Drug Administration (FDA). This laboratory is regulated under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.
Specimen must be centrifuged and plasma removed to another tube within two hours of collection. Centrifuge at 3,400 RPM's for 10 minutes. Follow directions in the centrifuge manual to set the centrifuge appropriately. Transfer the plasma using a disposable pipette or pouring to another labeled tube (pour off tube). Rubber band the original tube (parent tube) with the labeled pour off tube prior to sending the specimen to CLS/Immunology Lab. Separated plasma must be stored at -80C if Clinical Immunology Lab is closed.
Unacceptable Conditions
Severely hemolyzed and/ or grossly lipemic samples. Any specimen that has NOT been centrifuged and split within 2 hours of collection.
Storage/Transport Temperature
Transport at room temperature.
Store separated plasma at -80C.
Stability (from collection to initiation)
Sample is stable in PST tube for 2 hours. Centrifuge and remove plasma to another tube (pour off tube) within two hours of collection.
Remarks
Panel includes:
Interferon gamma (IFN-g)
Interleukin 1 beta (IL-1b)
Interleukin 2 (IL-2)
Interleukin 4 (IL-4)
Interleukin 6 (IL-6)
Interleukin 8 (IL-8)
Interleukin 10 (IL-10)
Interleukin 12p70 (IL-12p70)
Interleukin 13 (IL-13)
Tumor Necrosis Factor alpha (TNF-a)
Volume Required
3.0 ml
Minimum Required
1.0 ml
Phlebotomy Draw
Yes
Ordering
Performing Lab
Immunology Lab
Performed
Minimum of 2 days/week.
Methodology
Electrochemiluminescence
Reported
3 days
Synonyms
Cytokine
Cytokines
Interferon gamma
IFN-g
Interleukin 1 beta
IL-1b
Interleukin 2
IL-2
Interleukin 4
IL-4
Interleukin 6
IL-6
Interleukin 8
IL-8
Interleukin 10
IL-10
Interleukin 12p70
IL-12p70
Interleukin 13
IL-13
Tumor Necrosis Factor alpha
TNF-a
LIS Mnemonic
CYTK
Available STAT
Yes - requires Medical Director approval & DIRT consult
Result Interpretation
Interpretive Data
Any deviations from specimen processing instructions may cause unexpected results in all cytokines. Specifically for Interleukin 8 (IL-8), significantly falsely increased results may be observed.
This test was developed and its performance characteristics determined by the Clinical Immunology Laboratory of The Children's Hospital of Philadelphia. It has not been cleared or approved by the US Food and Drug Administration (FDA). This laboratory is regulated under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.
Reference Ranges
Analyte
Normal range (pg/ml)
IFN gamma
< 6.5
IL-1 beta
< 2.5
IL-2
< 6.5
IL-4
< 1.5
IL-6
< 3.5
IL-8
< 10.0
IL-10
< 2.0
IL-12 p70
< 2.5
IL-13
< 2.5
TNF alpha
<3.5
Administrative
CPT Codes
83520 x9, 83529 (IL-6)
Interpretive Data
Any deviations from specimen processing instructions may cause unexpected results in all cytokines. Specifically for Interleukin 8 (IL-8), significantly falsely increased results may be observed.
This test was developed and its performance characteristics determined by the Clinical Immunology Laboratory of The Children's Hospital of Philadelphia. It has not been cleared or approved by the US Food and Drug Administration (FDA). This laboratory is regulated under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.
