Acceptable:
Serum: Red Top (No additives)
Plasma: Sodium Heparin (NaHep/Green Top)
Preferred non-fasting sample, but fasting samples are accepted.
Specimen Preparation
On Site:
Specimen must be transported to the Metabolic lab within 2h of draw.
Preferred: Specimen should be on ice/refrigerated within 2h of draw.
Acceptable: Specimen should be on ice/refrigerated and arrive within 6h of draw or at room temperature and arrive within 2h of draw.
Off site:
Preferred: Specimen should be spun down within 2h of draw when transported on ice/refrigerated.
Acceptable: Specimen should be spun down within 6h of draw when transported on ice/refrigerated or within 2h of draw when transported at room temperature.
Non-CHOP Clients:
Preferred: Specimen should be spun down within 2h of draw and Plasma/Serum removed and frozen in separate tube. Specimen needs to be frozen when transported on dry ice.
Acceptable: Specimen should be spun down within 6h of draw and Plasma/Serum removed and frozen in separate tube. Specimen needs to be frozen when transported on dry ice and transported overnight.
Unacceptable Conditions
1. Receive whole blood specimen at room temperature after 2 hours of collection
2. Receive whole blood specimen refrigerated/on ice after 6 hours of collection
3. Receive separated serum/plasma at room temperature
4. Receive separated serum/plasma refrigerated (not frozen)
5. Insufficient sample volume
6. Improper sample handling and transport
7. Any other body fluid not listed above
8. Contaminated specimen
Storage/Transport Temperature
On Ice
Stability (from collection to initiation)
Room temperature: 2 hours
Refrigerated at 4C / on ice: 6 hours
Frozen in -20C for 5 days
Volume Required
2mL SST (gold) or Red top or Dark Green (NaHeparin)
Minimum Required
1mL SST (gold), Red top or Dark Green (NaHeparin)
Phlebotomy Draw
Yes
Performing Lab
Metabolic & Advanced Diagnostics Lab
Performed
Weekly
Methodology
LC-MS/MS
Synonyms
AcAc
Acetoacetate
BHB
Beta hydroxybutyrate
AHB
Alpha hydroxybutyrate
YHB
BHIB
Gamma hydroxybutyrate
Beta hydroxyisobutyrate
Ketone Body Panel
KBP
LIS Mnemonic
KBP
Available STAT
No
Reference Ranges
Acetoacetate (AcAc) 0.0072-0.2409 mM Beta-hydroxybutyrate (3OHB/BHB) 0.0078-0.4101 mM Ratio BHB/AcAc 0.4757-2.5654 Alpha-hydroxybutyrate (2OHB/AHB) 0.0106-0.1031 mM beta-hydroxyisobutyrate (3OHIB/BHIB) <0.0050-0.0439 mM Gamma-hydroxybutyrate (4OHB/YHB) <0.0025 mM
CPT Codes
82010
Billing (EAP) Codes
808284
Collection
Collect
Preferred:
Serum: Serum Separator Tube (SST/Gold)
Acceptable:
Serum: Red Top (No additives)
Plasma: Sodium Heparin (NaHep/Green Top)
Preferred non-fasting sample, but fasting samples are accepted.
Specimen Preparation
On Site:
Specimen must be transported to the Metabolic lab within 2h of draw.
Preferred: Specimen should be on ice/refrigerated within 2h of draw.
Acceptable: Specimen should be on ice/refrigerated and arrive within 6h of draw or at room temperature and arrive within 2h of draw.
Off site:
Preferred: Specimen should be spun down within 2h of draw when transported on ice/refrigerated.
Acceptable: Specimen should be spun down within 6h of draw when transported on ice/refrigerated or within 2h of draw when transported at room temperature.
Non-CHOP Clients:
Preferred: Specimen should be spun down within 2h of draw and Plasma/Serum removed and frozen in separate tube. Specimen needs to be frozen when transported on dry ice.
Acceptable: Specimen should be spun down within 6h of draw and Plasma/Serum removed and frozen in separate tube. Specimen needs to be frozen when transported on dry ice and transported overnight.
Unacceptable Conditions
1. Receive whole blood specimen at room temperature after 2 hours of collection
2. Receive whole blood specimen refrigerated/on ice after 6 hours of collection
3. Receive separated serum/plasma at room temperature
4. Receive separated serum/plasma refrigerated (not frozen)
5. Insufficient sample volume
6. Improper sample handling and transport
7. Any other body fluid not listed above
8. Contaminated specimen
Storage/Transport Temperature
On Ice
Stability (from collection to initiation)
Room temperature: 2 hours
Refrigerated at 4C / on ice: 6 hours
Frozen in -20C for 5 days
Volume Required
2mL SST (gold) or Red top or Dark Green (NaHeparin)
Minimum Required
1mL SST (gold), Red top or Dark Green (NaHeparin)
Phlebotomy Draw
Yes
Ordering
Performing Lab
Metabolic & Advanced Diagnostics Lab
Performed
Weekly
Methodology
LC-MS/MS
Synonyms
AcAc
Acetoacetate
BHB
Beta hydroxybutyrate
AHB
Alpha hydroxybutyrate
YHB
BHIB
Gamma hydroxybutyrate
Beta hydroxyisobutyrate
Ketone Body Panel
KBP
LIS Mnemonic
KBP
Available STAT
No
Result Interpretation
Reference Ranges
Acetoacetate (AcAc) 0.0072-0.2409 mM Beta-hydroxybutyrate (3OHB/BHB) 0.0078-0.4101 mM Ratio BHB/AcAc 0.4757-2.5654 Alpha-hydroxybutyrate (2OHB/AHB) 0.0106-0.1031 mM beta-hydroxyisobutyrate (3OHIB/BHIB) <0.0050-0.0439 mM Gamma-hydroxybutyrate (4OHB/YHB) <0.0025 mM
