Immediately transfer 400uL of blood collected in EDTA to a 10 mL polypropylene screwcap tube containing 3.6 mL of 32mM NEM, 3.8% SSA, 20% methanol (i.e. the crash tube).
CHOP Staff: Tube should be obtained from Central Processing or the Metabolic & Advanced Diagnostic lab.
External Clients: Please refer to this page to order a Glutathione Specimen Kit. Tubes are stable for 3 months from date of preparation, please use by the expiration date printed on the tube.
Kit Contents:
10mL polypropylene screwcap tube with 3.6 mL of 32mM N-Ethylmaleimide (NEM), 3.8% Sulfosalicylic Acid (SSA), 20% methanol (Please store tube at 4°C and protect from light until ready to use)
Ice pack
Fixed volume (0.4 mL) dual bulb pastette
Biohazard Bag with absorbent paper
Transport box
Compact cooler
Labels: UPS Return Label, Exempt Human Specimen, Biohazard, and Class 9 dry ice
Forms: Specimen Collection Procedure, Metabolic Lab Requisition
Unacceptable Conditions
Any sample collected in a tube other than EDTA.
Whole blood specimen crashed after 2 hours of the collection time.
Storage/Transport Temperature
*Note: These instructions are for collection of specimens only. Instructions for labeling collection container(s) are found in the hospital specimen labeling procedure.*
1. Primary specimen may be left at room temperature (NOT 4°C) for up to two hours after collection.
2. Transfer 0.4 mL of blood to a crash tube. Ideally, this should occur immediately after draw. As noted above, the maximum time before transferring the primary sample to the crash tube is 2 hours, and the primary tube should be stored at room temperature (NOT 4°C) prior to transfer.
3. Cap tube and mix immediately and thoroughly by repeatedly inverting the tube (6 times).
4. Allow the crash tube to sit at room temperature for 15 minutes.
5. Send the filled crash tube back to the laboratory on dry ice. It can be stored frozen at -80°C after blood has been crashed.
External Clients: A Specimen Collection form is included in the Glutathione kit and can be found here as well. Follow the instructions on the form to collect the sample and ship it back to CHOP.
Stability (from collection to initiation)
Stable up to 2 hours at room temperature.
Stable up to 1 year at -80C after blood is crashed in Glutathione Tube.
Minimum Required
1mL of Whole Blood in EDTA Tube
Ordering Recommendations
GSX / GSH / GSSG
Performing Lab
Metabolic and Advanced Diagnostic Laboratory
Methodology
UPLC-MS/MS
Reported
14 days
Available STAT
No
Reflex Testing
N/A
Vendor
None
CPT Codes
82978
Billing (EAP) Codes
808137
Collection
Collect
Preferred: 2mL Blood collected in EDTA tube.
Specimen Preparation
Immediately transfer 400uL of blood collected in EDTA to a 10 mL polypropylene screwcap tube containing 3.6 mL of 32mM NEM, 3.8% SSA, 20% methanol (i.e. the crash tube).
CHOP Staff: Tube should be obtained from Central Processing or the Metabolic & Advanced Diagnostic lab.
External Clients: Please refer to this page to order a Glutathione Specimen Kit. Tubes are stable for 3 months from date of preparation, please use by the expiration date printed on the tube.
Kit Contents:
10mL polypropylene screwcap tube with 3.6 mL of 32mM N-Ethylmaleimide (NEM), 3.8% Sulfosalicylic Acid (SSA), 20% methanol (Please store tube at 4°C and protect from light until ready to use)
Ice pack
Fixed volume (0.4 mL) dual bulb pastette
Biohazard Bag with absorbent paper
Transport box
Compact cooler
Labels: UPS Return Label, Exempt Human Specimen, Biohazard, and Class 9 dry ice
Forms: Specimen Collection Procedure, Metabolic Lab Requisition
Unacceptable Conditions
Any sample collected in a tube other than EDTA.
Whole blood specimen crashed after 2 hours of the collection time.
Storage/Transport Temperature
*Note: These instructions are for collection of specimens only. Instructions for labeling collection container(s) are found in the hospital specimen labeling procedure.*
1. Primary specimen may be left at room temperature (NOT 4°C) for up to two hours after collection.
2. Transfer 0.4 mL of blood to a crash tube. Ideally, this should occur immediately after draw. As noted above, the maximum time before transferring the primary sample to the crash tube is 2 hours, and the primary tube should be stored at room temperature (NOT 4°C) prior to transfer.
3. Cap tube and mix immediately and thoroughly by repeatedly inverting the tube (6 times).
4. Allow the crash tube to sit at room temperature for 15 minutes.
5. Send the filled crash tube back to the laboratory on dry ice. It can be stored frozen at -80°C after blood has been crashed.
External Clients: A Specimen Collection form is included in the Glutathione kit and can be found here as well. Follow the instructions on the form to collect the sample and ship it back to CHOP.
Stability (from collection to initiation)
Stable up to 2 hours at room temperature.
Stable up to 1 year at -80C after blood is crashed in Glutathione Tube.
