Collect

CHOP Patient Samples: 5 mL blood in Red Top Tube
External Client Samples: 2 mL frozen serum in freezer proof plastic tube

Specimen Preparation

CHOP Patient Samples: Transport 5 mL whole blood in red top tube to Central Lab Services (CLS). CLS will separate serum, place back in original vacutainer, refrigerate (or freeze if storage exceeds 24 hrs), and transport to Anatomic Pathology next business day.

External Client Samples: Separate serum, freeze in freezer proof plastic tube, and transport 2 mL frozen serum on dry ice to CHOP Anatomic Pathology; see also Handling Instructions on our website: https://www.chop.edu/centers-programs/division-anatomic-pathology

Unacceptable Conditions

CHOP Patient Samples: Whole blood held at room temperature > 2 hours, refrigerated > 1 week, or frozen. Specimen unlabeled, mislabeled, not ordered appropriately.

External Client Samples: Whole blood. Serum specimen not frozen. Specimen unlabeled, mislabeled, not ordered appropriately.

Storage/Transport Temperature

CHOP Patient Samples: Deliver to Central Lab Services immediately for processing to separate out the serum. The serum should then be refrigerated/frozen, if received after hours, holidays or weekends; during normal working hours take to Pathology immediately.

External Client Samples: Centrifuge and separate serum from cells and freeze. Ship frozen serum on dry ice so it remains frozen. See also Anti-Enterocyte Handling Instructions at https://www.chop.edu/centers-programs/division-anatomic-pathology

Stability (from collection to initiation)

CHOP Patient Samples: Whole blood - Room temperature: 2 hours; Refrigerated: 1 week

External Client samples: Serum - Frozen: indefinite

Volume Required

CHOP Patient Samples: 5 mL blood

External Client Samples: 2 mL serum

Minimum Required

CHOP Patient Samples: 3 mL blood

External Client Samples: 1 mL serum

Phlebotomy Draw

Yes

Clinical Features

To identify patients with circulating anti-enterocyte antibodies indicative of autoimmune enteropathy.

Ordering Recommendations

CHOP Providers: In Epic, order ANTI-ENTEROCYTE ANTIBODIES [88347.015]

External Clients: Complete Anti-Enterocyte Antibody Requisition & Anti-Enterocyte Antibody Clinical Form, which are available along with Handling instructions online at https://www.chop.edu/centers-programs/division-anatomic-pathology

Performing Lab

Anatomic Pathology

Performed

Batched weekly

Methodology

Indirect immunofluorescence

Reported

≥90% within 7 business days

LIS Mnemonic

SER

Available STAT

No

CPT Codes

88346
88350 x 2

Billing (EAP) Codes

800748
807167 x 2
Collection

Collect

CHOP Patient Samples: 5 mL blood in Red Top Tube
External Client Samples: 2 mL frozen serum in freezer proof plastic tube

Specimen Preparation

CHOP Patient Samples: Transport 5 mL whole blood in red top tube to Central Lab Services (CLS). CLS will separate serum, place back in original vacutainer, refrigerate (or freeze if storage exceeds 24 hrs), and transport to Anatomic Pathology next business day.

External Client Samples: Separate serum, freeze in freezer proof plastic tube, and transport 2 mL frozen serum on dry ice to CHOP Anatomic Pathology; see also Handling Instructions on our website: https://www.chop.edu/centers-programs/division-anatomic-pathology

Unacceptable Conditions

CHOP Patient Samples: Whole blood held at room temperature > 2 hours, refrigerated > 1 week, or frozen. Specimen unlabeled, mislabeled, not ordered appropriately.

External Client Samples: Whole blood. Serum specimen not frozen. Specimen unlabeled, mislabeled, not ordered appropriately.

Storage/Transport Temperature

CHOP Patient Samples: Deliver to Central Lab Services immediately for processing to separate out the serum. The serum should then be refrigerated/frozen, if received after hours, holidays or weekends; during normal working hours take to Pathology immediately.

External Client Samples: Centrifuge and separate serum from cells and freeze. Ship frozen serum on dry ice so it remains frozen. See also Anti-Enterocyte Handling Instructions at https://www.chop.edu/centers-programs/division-anatomic-pathology

Stability (from collection to initiation)

CHOP Patient Samples: Whole blood - Room temperature: 2 hours; Refrigerated: 1 week

External Client samples: Serum - Frozen: indefinite

Volume Required

CHOP Patient Samples: 5 mL blood

External Client Samples: 2 mL serum

Minimum Required

CHOP Patient Samples: 3 mL blood

External Client Samples: 1 mL serum

Phlebotomy Draw

Yes
Ordering

Clinical Features

To identify patients with circulating anti-enterocyte antibodies indicative of autoimmune enteropathy.

Ordering Recommendations

CHOP Providers: In Epic, order ANTI-ENTEROCYTE ANTIBODIES [88347.015]

External Clients: Complete Anti-Enterocyte Antibody Requisition & Anti-Enterocyte Antibody Clinical Form, which are available along with Handling instructions online at https://www.chop.edu/centers-programs/division-anatomic-pathology

Performing Lab

Anatomic Pathology

Performed

Batched weekly

Methodology

Indirect immunofluorescence

Reported

≥90% within 7 business days

LIS Mnemonic

SER

Available STAT

No
Result Interpretation
Administrative

CPT Codes

88346
88350 x 2

Billing (EAP) Codes

800748
807167 x 2