Collect

  • Bone marrow and leukemic blood samples: 3-5 ml in an EDTA (purple-top) tube.
  • Fresh Tissue 150 mg or 0.5-2.0 cm3 fresh tissue in transport media. Deliver within 24 hours at room temperature.
  • Fresh Frozen Tissue 150 mg or 0.5-2.0 cm3 tissue snap frozen in liquid nitrogen. Store at -80°C and prevent thawing. Ship on minimum of 10 lbs. of dry ice in an insulated container by overnight courier.
  • Formalin-fixed, paraffin embedded (FFPE) tissue: block of five 20-um scrolls that are freshly cut before shipping to us via overnight delivery. It would be best to cut and ship Monday to be delivered to us on Tuesday. Please note: Unlike the other specimens in which isolated RNA is extracted, only Total Nucleic Acid (DNA and RNA) is extracted from FFPE tissue.

Unacceptable Conditions

Unacceptable conditions include no tumor in tissue or specimens fixed/processed in alternative fixatives.

Storage/Transport Temperature

See above

Performed

Monday through Friday 9:00am - 4:00pm

Volume Required

See above

Minimum Required

See above or call the lab

Phlebotomy Draw

Yes

Performed

Monday through Friday 9:00am - 4:00pm

Methodology

Anchored multiplex PCR followed by Massively Parallel Sequencing. Real Time PCR may be used when needed.

Reported

21 days

Synonyms

  • Gene Fusion, Cancer Fusion, Rearrangements
  • FUSIP
  • ABL1, ABL2, AKT3, ALK, ARHGAP26, AXL, BCL2, BCL6, BCR, BRAF, BRD3, BRD4, CAMTA1, CBFB, CCNB3, CCND1, CIC, CRFL2, CSF1R, DNAJB1, DUSP22, EGFR, EPC1, EPOR, ERG, ESR1, ESRRA, ETV1, ETV4, ETV5, ETV6, EWSR1, FGFR1, FGFR2, FGFR3, FGR, FOXO1, FUS, GLI1, GLIS2, HMGA2, IL2RB, IL3, INSR, JAK2, JAZF1, KMT2A, MALT1, MAML2, MAST1, MAST2, MEAF6, MECOM, MET, MKL1, MKL2, MSMB, MUSK, MTB, MYC
  • NCOA2, NOTCH1, NOTCH2, NRG1, NTRK1, NTRK2, NTRK3, NUMBL, NUP214, NUP98, NUT, PAX5, PDGFB, PDGFRA, PDGFRB, PICALM, PIK3CA, PKN1, PLAG1, PPARG, PRKACA, PRKCA, PRKCB, PTK2B, RAF1, RARA, RBM15, RELA, RET, ROS1, RSPO2, RSPO3, RUNX1, RUNX1T1, SS18, STAT6, TAF15, TAL1, TCF12, TCF3, TERT, TFE3, TFEB, TFG, THADA, TMPRSS2, TSLP, TYK2, USP6, YWHAE

LIS Mnemonic

FUSIP

Performed By

Division of Genomic Diagnostics

Available STAT

No

Test Notes

NGS

Clinical Features

The CHOP Cancer Fusion Panel utilizes anchored multiplex PCR and next-generation sequencing (NGS) technologies to simultaneously analyze a panel of genes known to be involved in approximately 600 known cancer associated fusions. The CHOP Cancer Fusion Panel test can provide important fusions information which facilitates disease diagnosis, risk stratification, and therapeutic decision.

Molecular Testing Notes

110 fusion gene partners are analyzed for approximately 600 known fusions using anchored multiplex PCR technology followed by NGS. The technology allows detecting novel fusions associated with genes in this panel. Fusions are confirmed by FISH or Real Time PCR when necessary. This test does not detect fusions with no fusion partner included in this panel. The panel should not be used for detecting residual fusions at present time. The fusion partner genes included in this panel are listed below: ABL1, ABL2, AKT3, ALK, ARHGAP26, AXL, BCL2, BCL6, BCR, BRAF, BRD3, BRD4, CAMTA1, CBFB, CCNB3, CCND1, CIC, CRFL2, CSF1R, DNAJB1, DUSP22, EGFR, EPC1, EPOR, ERG, ESR1, ESRRA, ETV1, ETV4, ETV5, ETV6, EWSR1, FGFR1, FGFR2, FGFR3, FGR, FOXO1, FUS, GLI1, GLIS2, HMGA2, IL2RB, IL3, INSR, JAK2, JAZF1, KMT2A, MALT1, MAML2, MAST1, MAST2, MEAF6, MECOM, MET, MKL1, MKL2, MSMB, MUSK, MTB, MYC, NCOA2, NOTCH1, NOTCH2, NRG1, NTRK1, NTRK2, NTRK3, NUMBL, NUP214, NUP98, NUT, PAX5, PDGFB, PDGFRA, PDGFRB, PICALM, PIK3CA, PKN1, PLAG1, PPARG, PRKACA, PRKCA, PRKCB, PTK2B, RAF1, RARA, RBM15, RELA, RET, ROS1, RSPO2, RSPO3, RUNX1, RUNX1T1, SS18, STAT6, TAF15, TAL1, TCF12, TCF3, TERT, TFE3, TFEB, TFG, THADA, TMPRSS2, TSLP, TYK2, USP6, YWHAE

CPT Codes

81455
Collection

Collect

  • Bone marrow and leukemic blood samples: 3-5 ml in an EDTA (purple-top) tube.
  • Fresh Tissue 150 mg or 0.5-2.0 cm3 fresh tissue in transport media. Deliver within 24 hours at room temperature.
  • Fresh Frozen Tissue 150 mg or 0.5-2.0 cm3 tissue snap frozen in liquid nitrogen. Store at -80°C and prevent thawing. Ship on minimum of 10 lbs. of dry ice in an insulated container by overnight courier.
  • Formalin-fixed, paraffin embedded (FFPE) tissue: block of five 20-um scrolls that are freshly cut before shipping to us via overnight delivery. It would be best to cut and ship Monday to be delivered to us on Tuesday. Please note: Unlike the other specimens in which isolated RNA is extracted, only Total Nucleic Acid (DNA and RNA) is extracted from FFPE tissue.

Unacceptable Conditions

Unacceptable conditions include no tumor in tissue or specimens fixed/processed in alternative fixatives.

Storage/Transport Temperature

See above

Performed

Monday through Friday 9:00am - 4:00pm

Volume Required

See above

Minimum Required

See above or call the lab

Phlebotomy Draw

Yes
Ordering

Performed

Monday through Friday 9:00am - 4:00pm

Methodology

Anchored multiplex PCR followed by Massively Parallel Sequencing. Real Time PCR may be used when needed.

Reported

21 days

Synonyms

  • Gene Fusion, Cancer Fusion, Rearrangements
  • FUSIP
  • ABL1, ABL2, AKT3, ALK, ARHGAP26, AXL, BCL2, BCL6, BCR, BRAF, BRD3, BRD4, CAMTA1, CBFB, CCNB3, CCND1, CIC, CRFL2, CSF1R, DNAJB1, DUSP22, EGFR, EPC1, EPOR, ERG, ESR1, ESRRA, ETV1, ETV4, ETV5, ETV6, EWSR1, FGFR1, FGFR2, FGFR3, FGR, FOXO1, FUS, GLI1, GLIS2, HMGA2, IL2RB, IL3, INSR, JAK2, JAZF1, KMT2A, MALT1, MAML2, MAST1, MAST2, MEAF6, MECOM, MET, MKL1, MKL2, MSMB, MUSK, MTB, MYC
  • NCOA2, NOTCH1, NOTCH2, NRG1, NTRK1, NTRK2, NTRK3, NUMBL, NUP214, NUP98, NUT, PAX5, PDGFB, PDGFRA, PDGFRB, PICALM, PIK3CA, PKN1, PLAG1, PPARG, PRKACA, PRKCA, PRKCB, PTK2B, RAF1, RARA, RBM15, RELA, RET, ROS1, RSPO2, RSPO3, RUNX1, RUNX1T1, SS18, STAT6, TAF15, TAL1, TCF12, TCF3, TERT, TFE3, TFEB, TFG, THADA, TMPRSS2, TSLP, TYK2, USP6, YWHAE

LIS Mnemonic

FUSIP

Performed By

Division of Genomic Diagnostics

Available STAT

No

Test Notes

NGS

Clinical Features

The CHOP Cancer Fusion Panel utilizes anchored multiplex PCR and next-generation sequencing (NGS) technologies to simultaneously analyze a panel of genes known to be involved in approximately 600 known cancer associated fusions. The CHOP Cancer Fusion Panel test can provide important fusions information which facilitates disease diagnosis, risk stratification, and therapeutic decision.

Molecular Testing Notes

110 fusion gene partners are analyzed for approximately 600 known fusions using anchored multiplex PCR technology followed by NGS. The technology allows detecting novel fusions associated with genes in this panel. Fusions are confirmed by FISH or Real Time PCR when necessary. This test does not detect fusions with no fusion partner included in this panel. The panel should not be used for detecting residual fusions at present time. The fusion partner genes included in this panel are listed below: ABL1, ABL2, AKT3, ALK, ARHGAP26, AXL, BCL2, BCL6, BCR, BRAF, BRD3, BRD4, CAMTA1, CBFB, CCNB3, CCND1, CIC, CRFL2, CSF1R, DNAJB1, DUSP22, EGFR, EPC1, EPOR, ERG, ESR1, ESRRA, ETV1, ETV4, ETV5, ETV6, EWSR1, FGFR1, FGFR2, FGFR3, FGR, FOXO1, FUS, GLI1, GLIS2, HMGA2, IL2RB, IL3, INSR, JAK2, JAZF1, KMT2A, MALT1, MAML2, MAST1, MAST2, MEAF6, MECOM, MET, MKL1, MKL2, MSMB, MUSK, MTB, MYC, NCOA2, NOTCH1, NOTCH2, NRG1, NTRK1, NTRK2, NTRK3, NUMBL, NUP214, NUP98, NUT, PAX5, PDGFB, PDGFRA, PDGFRB, PICALM, PIK3CA, PKN1, PLAG1, PPARG, PRKACA, PRKCA, PRKCB, PTK2B, RAF1, RARA, RBM15, RELA, RET, ROS1, RSPO2, RSPO3, RUNX1, RUNX1T1, SS18, STAT6, TAF15, TAL1, TCF12, TCF3, TERT, TFE3, TFEB, TFG, THADA, TMPRSS2, TSLP, TYK2, USP6, YWHAE
Result Interpretation
Administrative

CPT Codes

81455