Collect

Bone marrow or leukemic blood sample in an EDTA (purple-top) tube.

Storage/Transport Temperature

Room Temperature

Performed

Monday-Friday 9:00am - 4:00pm

Volume Required

3-5 mL

Minimum Required

2 mL

Phlebotomy Draw

Yes

Performed

Monday-Friday 9:00am - 4:00pm

Reported

21 days

Synonyms

  • Somatic Leukemia Panel, Somatic Lymphoma Panel
  • HEMEP
  • ABL1, ASXL1, ASXL2, ATRX, BCL11B, BCL6, BCOR, BCORL1, BRAF, BRINP3, CALR, CBL, CCND3, CD79A, CD79B, CDC25C, CDKN2A, CDKN2B, CEBPA, CREBBP, CRLF2, CSF1R, CSF3R, CTCF, DDX41, DNM2, DNMT1, DNMT3A, DOT1L, EBF1, EED, ELANE, EP300, EPOR, ERG, ESR1, ETNK1, ETS1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GATA3, HNRNPK, HRAS, IDH1, IDH2, IKZF1, IKZF3, IL7R, JAK1, JAK2, JAK3, KDM6A, KIT, KMT2A, KMT2C, KMT2D, KRAS, LEF1, LYL1
  • MAP2K1, MPL, MSH2, MSH6, MYB, MYD88, NF1, NOTCH1, NPM1, NRAS, NSD1, NSD2 (WHSC1), NT5C2, PAX5, PDGFRA, PHF6, PIK3R1, PRPF40B, PRPF8, PTEN, PTPN11, RAD21, RB1, RELN, RPL10, RTEl1, RUNX1, SETBP1, SETD2, SF1, SF3A1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, SUZ12, TAL1, TCF3, TERT, TET2, TINF2, TLX1, TLX3, TP53, U2AF1, U2AF2, UBA2, USH2A, USP7, WT1, ZRSR2

LIS Mnemonic

HEMEP

Performed By

Division of Genomic Diagnostics

Available STAT

No

Test Notes

Targeted Capture followed by Massively Parallel Sequencing. MLPA, Real Time PCR, or aCGH may be used when needed.

Clinical Features

The CHOP Hematological Cancer Panel v2.2 utilizes next-generation sequencing technology to simultaneously analyze a panel of genes known to be associated with hematological malignancies. Hematological malignancies results from accumulation of multiple genomic alterations that lead to aberrant hematopoietic proliferation and differentiation. These genomic alterations are important diagnostic, prognostic, and therapeutic biologic markers. The CHOP Hematological Cancer Panel test can provide important genomic information regarding tumor development and progression, which facilitates more accurate disease diagnosis, risk stratification, and therapeutic decision.

Molecular Testing Notes

118 genes known to be associated with hematologic cancer are analyzed using Next Generation Sequence (NGS) technology. All coding exons of the 118 genes and 5 base pairs of 5' and 3' flanking intronic sequences are analyzed. Certain known intronic mutations of these genes are also evaluated. Clinically actionable or potentially actionable variants detected by NGS are confirmed by Sanger sequencing or other methodologies. The genes included in this panel are listed below: ABL1, ASXL1, ASXL2, ATRX, BCL11B, BCL6, BCOR, BCORL1, BRAF, BRINP3, CALR, CBL, CCND3, CD79A, CD79B, CDC25C, CDKN2A, CDKN2B, CEBPA, CREBBP, CRLF2, CSF1R, CSF3R, CTCF, DDX41, DNM2, DNMT1, DNMT3A, DOT1L, EBF1, EED, ELANE, EP300, EPOR, ERG, ESR1, ETNK1, ETS1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GATA3, HNRNPK, HRAS, IDH1, IDH2, IKZF1, IKZF3, IL7R, JAK1, JAK2, JAK3, KDM6A, KIT, KRAS, LEF1, LYL1, KMT2A, KMT2C, KMT2D, MAP2K1, MPL, MSH2, MSH6, MYB, MYD88, NF1, NOTCH1, NPM1, NRAS, NSD1, NT5C2, NUDT15, PAX5, PDGFRA, PHF6, PIK3R1, PRPF40B, PRPF8, PTEN, PTPN11, RAD21, RB1, RELN, RPL10, RETEI1, RUNX1, SETBP1, SETD2, SF1, SF3A1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, SUZ12, TAL1, TCF3, TERT, TET2, TINF2, TLX1, TLX3, TP53, TPMT, U2AF1, U2AF2, UBA2, USH2A, USP7, WHSC1, WT1, ZRSR2

CPT Codes

81455
Collection

Collect

Bone marrow or leukemic blood sample in an EDTA (purple-top) tube.

Storage/Transport Temperature

Room Temperature

Performed

Monday-Friday 9:00am - 4:00pm

Volume Required

3-5 mL

Minimum Required

2 mL

Phlebotomy Draw

Yes
Ordering

Performed

Monday-Friday 9:00am - 4:00pm

Reported

21 days

Synonyms

  • Somatic Leukemia Panel, Somatic Lymphoma Panel
  • HEMEP
  • ABL1, ASXL1, ASXL2, ATRX, BCL11B, BCL6, BCOR, BCORL1, BRAF, BRINP3, CALR, CBL, CCND3, CD79A, CD79B, CDC25C, CDKN2A, CDKN2B, CEBPA, CREBBP, CRLF2, CSF1R, CSF3R, CTCF, DDX41, DNM2, DNMT1, DNMT3A, DOT1L, EBF1, EED, ELANE, EP300, EPOR, ERG, ESR1, ETNK1, ETS1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GATA3, HNRNPK, HRAS, IDH1, IDH2, IKZF1, IKZF3, IL7R, JAK1, JAK2, JAK3, KDM6A, KIT, KMT2A, KMT2C, KMT2D, KRAS, LEF1, LYL1
  • MAP2K1, MPL, MSH2, MSH6, MYB, MYD88, NF1, NOTCH1, NPM1, NRAS, NSD1, NSD2 (WHSC1), NT5C2, PAX5, PDGFRA, PHF6, PIK3R1, PRPF40B, PRPF8, PTEN, PTPN11, RAD21, RB1, RELN, RPL10, RTEl1, RUNX1, SETBP1, SETD2, SF1, SF3A1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, SUZ12, TAL1, TCF3, TERT, TET2, TINF2, TLX1, TLX3, TP53, U2AF1, U2AF2, UBA2, USH2A, USP7, WT1, ZRSR2

LIS Mnemonic

HEMEP

Performed By

Division of Genomic Diagnostics

Available STAT

No

Test Notes

Targeted Capture followed by Massively Parallel Sequencing. MLPA, Real Time PCR, or aCGH may be used when needed.

Clinical Features

The CHOP Hematological Cancer Panel v2.2 utilizes next-generation sequencing technology to simultaneously analyze a panel of genes known to be associated with hematological malignancies. Hematological malignancies results from accumulation of multiple genomic alterations that lead to aberrant hematopoietic proliferation and differentiation. These genomic alterations are important diagnostic, prognostic, and therapeutic biologic markers. The CHOP Hematological Cancer Panel test can provide important genomic information regarding tumor development and progression, which facilitates more accurate disease diagnosis, risk stratification, and therapeutic decision.

Molecular Testing Notes

118 genes known to be associated with hematologic cancer are analyzed using Next Generation Sequence (NGS) technology. All coding exons of the 118 genes and 5 base pairs of 5' and 3' flanking intronic sequences are analyzed. Certain known intronic mutations of these genes are also evaluated. Clinically actionable or potentially actionable variants detected by NGS are confirmed by Sanger sequencing or other methodologies. The genes included in this panel are listed below: ABL1, ASXL1, ASXL2, ATRX, BCL11B, BCL6, BCOR, BCORL1, BRAF, BRINP3, CALR, CBL, CCND3, CD79A, CD79B, CDC25C, CDKN2A, CDKN2B, CEBPA, CREBBP, CRLF2, CSF1R, CSF3R, CTCF, DDX41, DNM2, DNMT1, DNMT3A, DOT1L, EBF1, EED, ELANE, EP300, EPOR, ERG, ESR1, ETNK1, ETS1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GATA3, HNRNPK, HRAS, IDH1, IDH2, IKZF1, IKZF3, IL7R, JAK1, JAK2, JAK3, KDM6A, KIT, KRAS, LEF1, LYL1, KMT2A, KMT2C, KMT2D, MAP2K1, MPL, MSH2, MSH6, MYB, MYD88, NF1, NOTCH1, NPM1, NRAS, NSD1, NT5C2, NUDT15, PAX5, PDGFRA, PHF6, PIK3R1, PRPF40B, PRPF8, PTEN, PTPN11, RAD21, RB1, RELN, RPL10, RETEI1, RUNX1, SETBP1, SETD2, SF1, SF3A1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, SUZ12, TAL1, TCF3, TERT, TET2, TINF2, TLX1, TLX3, TP53, TPMT, U2AF1, U2AF2, UBA2, USH2A, USP7, WHSC1, WT1, ZRSR2
Result Interpretation
Administrative

CPT Codes

81455