Bone marrow or leukemic blood sample in an EDTA (purple-top) tube.
Fresh Tissue 150 mg or 0.5-2.0 cm3 fresh tissue in transport media. Deliver within 24 hours at room temperature.
Fresh Frozen Tissue 150 mg or 0.5-2.0 cm3 tissue snap frozen at -20°C. Store at -20°C. Ship on minimum of 10 lbs. of dry ice in an insulated container by overnight courier.
FFPE preferred sample: minimum three 20-um scrolls or 5 slides that are freshly cut before shipping to us via overnight delivery. It would be best to cut and ship Monday to be delivered to us on Tuesday. Alternatively, a whole FFPE block can be sent.
Unacceptable Conditions
Heparinized specimens, severely hemolyzed specimens, frozen, clotted or possibly commingled specimens, blood in non-sterile or leaky containers, mislabeled or inappropriately labeled specimens.
Storage/Transport Temperature
For CHOP Phlebotomy: Samples can be collected throughout the week. Samples collected on weekends or holidays are held in Central Labs and sent to the Genomic Diagnostic Lab the following business day.
For External Clients: Refrigerate sample until shipment. Send the sample at room temperature with overnight delivery for receipt Monday through Friday, optimally within 24 hours of collection.
Please contact the lab (267-426-1447) with questions regarding non-blood specimens.
Volume Required
3-5 mL
Minimum Required
2 mL
Phlebotomy Draw
Yes
Performing Lab
Division of Genomic Diagnostics
Performed
Monday-Friday 9:00am - 4:00pm
Reported
21 days
Detection Rate
The Hematological Cancer Panel V2.3 includes sequence and copy number analyses of 117 cancer genes and two genes associated with cancer pharmacogenomics.
Utility
Facilitates disease diagnosis, risk stratification, and therapeutic decision-making.
Hematologic, Heme, Hematologic Panel, Heme Panel, Hematologic Cancer Panel, Heme Cancer Panel
LIS Mnemonic
HEMEP
Available STAT
Yes
Test Notes
Next generation sequencing (NGS) and data analysis: Genomic DNA is extracted from the patient’s sample following standard DNA extraction protocols. Extracted DNA is fragmented and tagged using SureSelect QXT target enrichment to generate adapter-tagged libraries. Biotin-labeled probes specific to the targeted regions are used for capture hybridization. Libraries are enriched for the desired regions using streptavidin beads. Enriched libraries are then indexed and pooled for sequencing. Libraries are subject to sequence analysis on Illumina NovaSeq 6000 system for 150 bp paired end reads. All coding exons and the flanking intron sequences of targeted genes in the panel are sequenced, and selected promoter regions and known intronic mutations are also evaluated. Sequence data are analyzed using the home brew software ConcordS V4.0.0 and NextGENe V2 NGS Analysis Software. Sequence variants within exons and 5 bp flanking intron sequences are annotated. Copy number variation (CNV) analysis for gross deletions and duplications are evaluated using NGS data. Clinically significant variants including single nucleotide variants (SNVs), indels, and CNVs are confirmed by Sanger sequencing, MLPA, Real-Time PCR, or ddPCR only when necessary.
Bone marrow or leukemic blood sample in an EDTA (purple-top) tube.
Fresh Tissue 150 mg or 0.5-2.0 cm3 fresh tissue in transport media. Deliver within 24 hours at room temperature.
Fresh Frozen Tissue 150 mg or 0.5-2.0 cm3 tissue snap frozen at -20°C. Store at -20°C. Ship on minimum of 10 lbs. of dry ice in an insulated container by overnight courier.
FFPE preferred sample: minimum three 20-um scrolls or 5 slides that are freshly cut before shipping to us via overnight delivery. It would be best to cut and ship Monday to be delivered to us on Tuesday. Alternatively, a whole FFPE block can be sent.
Unacceptable Conditions
Heparinized specimens, severely hemolyzed specimens, frozen, clotted or possibly commingled specimens, blood in non-sterile or leaky containers, mislabeled or inappropriately labeled specimens.
Storage/Transport Temperature
For CHOP Phlebotomy: Samples can be collected throughout the week. Samples collected on weekends or holidays are held in Central Labs and sent to the Genomic Diagnostic Lab the following business day.
For External Clients: Refrigerate sample until shipment. Send the sample at room temperature with overnight delivery for receipt Monday through Friday, optimally within 24 hours of collection.
Please contact the lab (267-426-1447) with questions regarding non-blood specimens.
Volume Required
3-5 mL
Minimum Required
2 mL
Phlebotomy Draw
Yes
Ordering
Performing Lab
Division of Genomic Diagnostics
Performed
Monday-Friday 9:00am - 4:00pm
Reported
21 days
Detection Rate
The Hematological Cancer Panel V2.3 includes sequence and copy number analyses of 117 cancer genes and two genes associated with cancer pharmacogenomics.
Utility
Facilitates disease diagnosis, risk stratification, and therapeutic decision-making.
Hematologic, Heme, Hematologic Panel, Heme Panel, Hematologic Cancer Panel, Heme Cancer Panel
LIS Mnemonic
HEMEP
Available STAT
Yes
Test Notes
Next generation sequencing (NGS) and data analysis: Genomic DNA is extracted from the patient’s sample following standard DNA extraction protocols. Extracted DNA is fragmented and tagged using SureSelect QXT target enrichment to generate adapter-tagged libraries. Biotin-labeled probes specific to the targeted regions are used for capture hybridization. Libraries are enriched for the desired regions using streptavidin beads. Enriched libraries are then indexed and pooled for sequencing. Libraries are subject to sequence analysis on Illumina NovaSeq 6000 system for 150 bp paired end reads. All coding exons and the flanking intron sequences of targeted genes in the panel are sequenced, and selected promoter regions and known intronic mutations are also evaluated. Sequence data are analyzed using the home brew software ConcordS V4.0.0 and NextGENe V2 NGS Analysis Software. Sequence variants within exons and 5 bp flanking intron sequences are annotated. Copy number variation (CNV) analysis for gross deletions and duplications are evaluated using NGS data. Clinically significant variants including single nucleotide variants (SNVs), indels, and CNVs are confirmed by Sanger sequencing, MLPA, Real-Time PCR, or ddPCR only when necessary.
