Patient Preparation

Specimens are to be collected from a site prepared using aseptic technique. Contamination with usual microbiota from skin, mouth, rectum, vagina, urinary tract orifices and other mucosal or skin surfaces must be avoided.

Collect

Container:   Anaerobe Systems Anaerobic tissue transport medium (preferred) 
 
Acceptable specimens: abdominal fluid, abscess, amniotic fluid, appendix, ascitic fluid, aspirate, biliary fluid, biopsy (tissue), bone, brain, cyst fluid, drainage, endometrium, fistula, graft, heart valve, lesion, liver, lung, lymph node, pelvic fluid, pericardial fluid, peritoneal fluid, pleura, pleural fluid, shunt fluid, sinus, synovial fluid, urine from suprapubic aspirate.

Recovery of anaerobes is often a function of the amount of specimen received. Avoid swabs whenever possible; they DO NOT result in optimal recovery of anaerobes.  Performance of the ESwabTM Liquid Amies Collection and Transport System that employs a flocked swab for recovery of anaerobes is better, but still less than optimal.  

It is imperative that the specimen be taken in a manner that prevents contamination by usual microbiota of surrounding areas.

Specimen Preparation

The preferred specimen is a tissue biopsy, body fluid aliquot, or pus obtained by needle aspiration. Aseptically remove any surface debris at wound sites. Contamination with usual microbiota around areas must be avoided. Some anerobes will be killed rapidly by contact with oxygen; therefore the specimen must be placed in an anaerobic transport container as soon as possible.
Open tube by screw cap and keep tube upright. Place tissue or fluid on the surface of the semisolid medium; inserting the tissue or fluid into the gel is not necessary. Immediately close the tube. Oxygen contact within the medium should be minimized. Once inoculated, keep at room temperaure.
 

DO NOT send needled syringe to lab.

Specimen must be transported to the Clinical Microbiology laboratory (ST-730) as soon as possible.

Unacceptable Conditions

Specimen not received in Anaerobe Systems Anaerobic tissue transport medium or BD ESwabTM  Transport System; specimens exposed to air; specimens that have been refrigerated; specimens from sites which have usual microbiota. If specimen is unacceptable, that information will be reported directly by computer. 

Storage/Transport Temperature

Do not refrigerate. Transport at room temperature.

Stability (from collection to initiation)

Use anaerobic transport medium. Send to laboratory immediately. If delay occurs, keep at room temperature. DO NOT refrigerate.

Performed

Daily

Remarks

Culture for anaerobes is NOT performed on specimens from sites with indigenous microbiota such as sputae and BALs, mucosal linings, vaginal or cervical swabs, intestinal contents, surface wounds, and nose, throat, or mouth specimens.

 

Notes

Microbiology (Bacteriology), ST-730

Phone Number: (212)746-2400

Collect

Container:   Anaerobe Systems Anaerobic tissue transport medium (preferred) 
 
Acceptable specimens: abdominal fluid, abscess, amniotic fluid, appendix, ascitic fluid, aspirate, biliary fluid, biopsy (tissue), bone, brain, cyst fluid, drainage, endometrium, fistula, graft, heart valve, lesion, liver, lung, lymph node, pelvic fluid, pericardial fluid, peritoneal fluid, pleura, pleural fluid, shunt fluid, sinus, synovial fluid, urine from suprapubic aspirate.

Recovery of anaerobes is often a function of the amount of specimen received. Avoid swabs whenever possible; they DO NOT result in optimal recovery of anaerobes.  Performance of the ESwabTM Liquid Amies Collection and Transport System that employs a flocked swab for recovery of anaerobes is better, but still less than optimal.  

It is imperative that the specimen be taken in a manner that prevents contamination by usual microbiota of surrounding areas.

Performed

Daily

Methodology

Culture

Reported

Cultures showing no bacterial growth can generally be reported after 5 days. Complete reports of cultures with anaerobic bacteria may take as long as 1 week after receipt of specimen, depending upon the nature of the organisms isolated.

Reference Interval

No anaerobes isolated.

CPT Codes

87075

CPT codes for identification and susceptibility vary based on method

Collection

Patient Preparation

Specimens are to be collected from a site prepared using aseptic technique. Contamination with usual microbiota from skin, mouth, rectum, vagina, urinary tract orifices and other mucosal or skin surfaces must be avoided.

Collect

Container:   Anaerobe Systems Anaerobic tissue transport medium (preferred) 
 
Acceptable specimens: abdominal fluid, abscess, amniotic fluid, appendix, ascitic fluid, aspirate, biliary fluid, biopsy (tissue), bone, brain, cyst fluid, drainage, endometrium, fistula, graft, heart valve, lesion, liver, lung, lymph node, pelvic fluid, pericardial fluid, peritoneal fluid, pleura, pleural fluid, shunt fluid, sinus, synovial fluid, urine from suprapubic aspirate.

Recovery of anaerobes is often a function of the amount of specimen received. Avoid swabs whenever possible; they DO NOT result in optimal recovery of anaerobes.  Performance of the ESwabTM Liquid Amies Collection and Transport System that employs a flocked swab for recovery of anaerobes is better, but still less than optimal.  

It is imperative that the specimen be taken in a manner that prevents contamination by usual microbiota of surrounding areas.

Specimen Preparation

The preferred specimen is a tissue biopsy, body fluid aliquot, or pus obtained by needle aspiration. Aseptically remove any surface debris at wound sites. Contamination with usual microbiota around areas must be avoided. Some anerobes will be killed rapidly by contact with oxygen; therefore the specimen must be placed in an anaerobic transport container as soon as possible.
Open tube by screw cap and keep tube upright. Place tissue or fluid on the surface of the semisolid medium; inserting the tissue or fluid into the gel is not necessary. Immediately close the tube. Oxygen contact within the medium should be minimized. Once inoculated, keep at room temperaure.
 

DO NOT send needled syringe to lab.

Specimen must be transported to the Clinical Microbiology laboratory (ST-730) as soon as possible.

Unacceptable Conditions

Specimen not received in Anaerobe Systems Anaerobic tissue transport medium or BD ESwabTM  Transport System; specimens exposed to air; specimens that have been refrigerated; specimens from sites which have usual microbiota. If specimen is unacceptable, that information will be reported directly by computer. 

Storage/Transport Temperature

Do not refrigerate. Transport at room temperature.

Stability (from collection to initiation)

Use anaerobic transport medium. Send to laboratory immediately. If delay occurs, keep at room temperature. DO NOT refrigerate.

Performed

Daily

Remarks

Culture for anaerobes is NOT performed on specimens from sites with indigenous microbiota such as sputae and BALs, mucosal linings, vaginal or cervical swabs, intestinal contents, surface wounds, and nose, throat, or mouth specimens.

 

Notes

Microbiology (Bacteriology), ST-730

Phone Number: (212)746-2400

Container

Collect

Container:   Anaerobe Systems Anaerobic tissue transport medium (preferred) 
 
Acceptable specimens: abdominal fluid, abscess, amniotic fluid, appendix, ascitic fluid, aspirate, biliary fluid, biopsy (tissue), bone, brain, cyst fluid, drainage, endometrium, fistula, graft, heart valve, lesion, liver, lung, lymph node, pelvic fluid, pericardial fluid, peritoneal fluid, pleura, pleural fluid, shunt fluid, sinus, synovial fluid, urine from suprapubic aspirate.

Recovery of anaerobes is often a function of the amount of specimen received. Avoid swabs whenever possible; they DO NOT result in optimal recovery of anaerobes.  Performance of the ESwabTM Liquid Amies Collection and Transport System that employs a flocked swab for recovery of anaerobes is better, but still less than optimal.  

It is imperative that the specimen be taken in a manner that prevents contamination by usual microbiota of surrounding areas.

Ordering

Performed

Daily

Methodology

Culture

Reported

Cultures showing no bacterial growth can generally be reported after 5 days. Complete reports of cultures with anaerobic bacteria may take as long as 1 week after receipt of specimen, depending upon the nature of the organisms isolated.

Result Interpretation

Reference Interval

No anaerobes isolated.

Administrative

CPT Codes

87075

CPT codes for identification and susceptibility vary based on method