If patient needs to be scheduled for an appointment for an ACTH Stimulation please contact Infusion Center located at Methodist Estabrook Cancer Center (MECC) 402-354-4379.
Collect
Draw 5 mL SST (Heparinized plasma is acceptable) for cortisol at 0, 30 and 60 minutes. If an ACTH level is also ordered, draw 4 mL lavendar (K2EDTA) at 0 minutes.
Pediatric Collection
0.5 mL serum or li-heparin plasma for Cortisol 0.5 mL EDTA plasma for ACTH
Storage/Transport Temperature
Cortisol: Minimum 1 mL serum or plasma per collection time. Refrigerate 2-8°C.
ACTH: Minimum 0.5 mL EDTA plasma in an ARUP Standard Transport Tube. Transport FROZEN.
Remarks
Order as individual Cortisol tests indicating time and date of draw. Baseline cortisol specimen need not be fasting but is recommended to be drawn 1 hour or less prior to injection of cortrosyn (0.25 mg). Post-injection specimens are drawn at 30 and 60 minutes.
NOTES: Samples should not be taken from patients receiving therapy with high dose biotin (i.e. > 5mg/dl) until at least 8 hours following the last biotin administration.
Interpretive Data
Interpretive data added to 30 and 60 minute results: Normal peak serum cortisol is greater than 20.0 ug/dL 30 to 60 minutes after 0.25 mg Cosyntopin IV or IM.
CPT Codes
82533 X 3
LOINC
2143-6
26437-4
2141-0
Includes
Cortisol baseline, Cortisol 30 min, Cortisol 60 min, and ACTH
Collection
Synonyms
ACTH Stimulation
Cortrosyn Stimulation
Cortisol ACTH
Corsyntropin Stimulation
Performed
Daily
Patient Preparation
If patient needs to be scheduled for an appointment for an ACTH Stimulation please contact Infusion Center located at Methodist Estabrook Cancer Center (MECC) 402-354-4379.
Collect
Draw 5 mL SST (Heparinized plasma is acceptable) for cortisol at 0, 30 and 60 minutes. If an ACTH level is also ordered, draw 4 mL lavendar (K2EDTA) at 0 minutes.
Pediatric Collection
0.5 mL serum or li-heparin plasma for Cortisol 0.5 mL EDTA plasma for ACTH
Storage/Transport Temperature
Cortisol: Minimum 1 mL serum or plasma per collection time. Refrigerate 2-8°C.
ACTH: Minimum 0.5 mL EDTA plasma in an ARUP Standard Transport Tube. Transport FROZEN.
Remarks
Order as individual Cortisol tests indicating time and date of draw. Baseline cortisol specimen need not be fasting but is recommended to be drawn 1 hour or less prior to injection of cortrosyn (0.25 mg). Post-injection specimens are drawn at 30 and 60 minutes.
NOTES: Samples should not be taken from patients receiving therapy with high dose biotin (i.e. > 5mg/dl) until at least 8 hours following the last biotin administration.
Interpretive Data
Interpretive data added to 30 and 60 minute results: Normal peak serum cortisol is greater than 20.0 ug/dL 30 to 60 minutes after 0.25 mg Cosyntopin IV or IM.
Administrative
CPT Codes
82533 X 3
LOINC
2143-6
26437-4
2141-0
Includes
Includes
Cortisol baseline, Cortisol 30 min, Cortisol 60 min, and ACTH
