Prior to Testing or Freezing, collection tubes must be double centrifuged at a time and speed that results in the production of platelet poor plasma which contains a residual platelet count of <10,000/uL.
Unacceptable Conditions
Serum. EDTA plasma, clotted or hemolyzed specimens &/or improperly labeled or filled collection tube.
Storage/Transport Temperature
Whole blood (Spun or Unspun) can be transported or stored at room temperature (18-24 °C) for up to 4 hours. Platelet Poor Plasma Separated from the cells can be transported or stored Frozen at -20°C for up to 2 weeks.
Stability (from collection to initiation)
Whole Blood (Spun or Unspun) stored at Room Temperature (18-24°C) for up to 4 hours.
Platelet Poor Plasma Separated from the cells and Frozen at -20°C for up to 2 weeks
Cerner Orderable(s)
Factor V Assay
Performed
St. Joseph's Hospital
Performed on day shift. Specimen must be received by 1100 am for same day result reporting.
Ordering Recommendations
Factor V is a Vitamin dependent protein synthesized in the liver, endothelium, by megakaryocytes, and platelets. It is activated by thrombin and is an essential cofactor in the prothrombinase enzyme complex which converts prothrombin to thrombin. Deficiencies may be congenital or acquired and can cause prolongation of both PT and APTT tests. The congenital deficiency leads to Owren's Disease which is a rare disorder that causes mild to severe bleeding. Acquired deficiency may be due to Liver Disease, Vitamin K deficiency, Factor V Inhibitors and disseminated intravascular coagulation (DIC).
Methodology
Clot Detection and analysis by Spectrophotometic Evaluation. The Factor V activity in patient's plasma is determined by performing a modified activated prothrombin time test (PT). Patient plasma is diluted and added to plasma deficient in factor V. Correction of the clotting time of the deficient plasma is proportional to the concentration (% activity) of that factor in the patient plasma. The final patient result (% activity) is interpolated from a calibration curve.
Reported
Within 24 hours
Synonyms
Plasma Accelerator Globulin
Proaccelerin
Proconvertin
Coagulation Factor V Activity Assay, Plasma
Common Pathway Proteins, Factor V
F5
Labile Factor
Reference Interval
Factor V Reference Interval: 70.0-140.0%
CPT Codes
85220
Collection
Collect
Preferred
Tube Name
Blue
Optimum Volume
Full
Minimum Volume
Full
Specimen Preparation
Prior to Testing or Freezing, collection tubes must be double centrifuged at a time and speed that results in the production of platelet poor plasma which contains a residual platelet count of <10,000/uL.
Unacceptable Conditions
Serum. EDTA plasma, clotted or hemolyzed specimens &/or improperly labeled or filled collection tube.
Storage/Transport Temperature
Whole blood (Spun or Unspun) can be transported or stored at room temperature (18-24 °C) for up to 4 hours. Platelet Poor Plasma Separated from the cells can be transported or stored Frozen at -20°C for up to 2 weeks.
Stability (from collection to initiation)
Whole Blood (Spun or Unspun) stored at Room Temperature (18-24°C) for up to 4 hours.
Platelet Poor Plasma Separated from the cells and Frozen at -20°C for up to 2 weeks
Ordering
Cerner Orderable(s)
Factor V Assay
Performed
St. Joseph's Hospital
Performed on day shift. Specimen must be received by 1100 am for same day result reporting.
Ordering Recommendations
Factor V is a Vitamin dependent protein synthesized in the liver, endothelium, by megakaryocytes, and platelets. It is activated by thrombin and is an essential cofactor in the prothrombinase enzyme complex which converts prothrombin to thrombin. Deficiencies may be congenital or acquired and can cause prolongation of both PT and APTT tests. The congenital deficiency leads to Owren's Disease which is a rare disorder that causes mild to severe bleeding. Acquired deficiency may be due to Liver Disease, Vitamin K deficiency, Factor V Inhibitors and disseminated intravascular coagulation (DIC).
Methodology
Clot Detection and analysis by Spectrophotometic Evaluation. The Factor V activity in patient's plasma is determined by performing a modified activated prothrombin time test (PT). Patient plasma is diluted and added to plasma deficient in factor V. Correction of the clotting time of the deficient plasma is proportional to the concentration (% activity) of that factor in the patient plasma. The final patient result (% activity) is interpolated from a calibration curve.
