Collect

 5 mL Whole Blood in Lavender or Pink Top (EDTA).
 

Pediatric Collection

 1 mL Whole Blood in Lavender or Pink Top (EDTA).

Unacceptable Conditions

Serum Separator Tube, Sample Frozen.

Storage/Transport Temperature

Transport: Room temperature
Store: Refrigerated
 

Stability (from collection to initiation)

Room Temparature: 72 hours
Refrigerated: 6 days
Frozen: Unacceptable

Remarks

Limitations:  Interferennces may include abnormal plasma proteins, cold autoagglutinins, positive antiglobulin test, and bacteremia.

Cerner Orderable(s)

BB ABO + RH

Performed

Bardmoor ER Laboratory, Bartow Regional Medical Center, Mease Countryside Hospital, Mease Dunedin Hospital, Morton Plant

Hospital, Morton Plant North Bay Hospital, South Florida Baptist Hospital, St. Anthony's Hospital, St. Joseph's Hospital, St. Joseph's

Hospital North, St. Joseph's Hospital South, Winter Haven Hospital.

Methodology

Immune Agglutination

Reported

ABO Typing: A, B, AB, O
Rh Typing: Rh positive/Rh negative

Reference Interval

ABO Typing: A, B, AB, O
Rh Typing: Rh positive/Rh negative

Reflex Algorithm

Type and Screen Requests may include all appropriate orders/tests outlined below and/or in Transfusion Services Procedures as needed:
ABO/Rh Type (ABO/Rh Repeat, ABO Confirm, Grp/Rh-OneBlood, 2nd ABO/Rh-OneBlood, Donor Confirm, Weak D, Historical ABO/Rh)  
Antibody Testing – Indirect Coombs Test (AB Screen-Tube/LISS, PeG, Albumin, Prewarm, AB ID-Tube/Solid Phase, AB ID-Elution, Enzyme Panel, Cold Panel, DTT, Add Panel, Pre-warm AB ID, Historical AB ID, Additional Sample Request
BB-free Anti-A screen, free anti-B screen)
DAT – Direct Coombs Test (Monospecific IgG, Monospecific C3b, C3d, Polyspecific IgG, C3b, C3d)
Antigen Typing (Antigen Type, Historical Antigen Type) RBC Genotype
Crossmatch (XM Electronic, XM IS, XM AHG, Pre-warm XM, XM AHG LISS, PeG, Albumin,
Uncrossmatched-Emergency Release)
Other (BB RBC Hgb S Testing, BB Antibody Titer, Platelet Antibody/Crossmatch, HLA Typing, Volume Reduction, CMV Required)

*Transfusion Reaction Testing may include all testing above  and TX Reaction Workup, Blood Culture/Gram Stain, Urine Hemoglobin, TRALI Investigation.

CPT Codes

86900, 86901
Collection

Collect

 5 mL Whole Blood in Lavender or Pink Top (EDTA).
 

Pediatric Collection

 1 mL Whole Blood in Lavender or Pink Top (EDTA).

Unacceptable Conditions

Serum Separator Tube, Sample Frozen.

Storage/Transport Temperature

Transport: Room temperature
Store: Refrigerated
 

Stability (from collection to initiation)

Room Temparature: 72 hours
Refrigerated: 6 days
Frozen: Unacceptable

Remarks

Limitations:  Interferennces may include abnormal plasma proteins, cold autoagglutinins, positive antiglobulin test, and bacteremia.
Ordering

Cerner Orderable(s)

BB ABO + RH

Performed

Bardmoor ER Laboratory, Bartow Regional Medical Center, Mease Countryside Hospital, Mease Dunedin Hospital, Morton Plant

Hospital, Morton Plant North Bay Hospital, South Florida Baptist Hospital, St. Anthony's Hospital, St. Joseph's Hospital, St. Joseph's

Hospital North, St. Joseph's Hospital South, Winter Haven Hospital.

Methodology

Immune Agglutination

Reported

ABO Typing: A, B, AB, O
Rh Typing: Rh positive/Rh negative
Result Interpretation

Reference Interval

ABO Typing: A, B, AB, O
Rh Typing: Rh positive/Rh negative

Reflex Algorithm

Type and Screen Requests may include all appropriate orders/tests outlined below and/or in Transfusion Services Procedures as needed:
ABO/Rh Type (ABO/Rh Repeat, ABO Confirm, Grp/Rh-OneBlood, 2nd ABO/Rh-OneBlood, Donor Confirm, Weak D, Historical ABO/Rh)  
Antibody Testing – Indirect Coombs Test (AB Screen-Tube/LISS, PeG, Albumin, Prewarm, AB ID-Tube/Solid Phase, AB ID-Elution, Enzyme Panel, Cold Panel, DTT, Add Panel, Pre-warm AB ID, Historical AB ID, Additional Sample Request
BB-free Anti-A screen, free anti-B screen)
DAT – Direct Coombs Test (Monospecific IgG, Monospecific C3b, C3d, Polyspecific IgG, C3b, C3d)
Antigen Typing (Antigen Type, Historical Antigen Type) RBC Genotype
Crossmatch (XM Electronic, XM IS, XM AHG, Pre-warm XM, XM AHG LISS, PeG, Albumin,
Uncrossmatched-Emergency Release)
Other (BB RBC Hgb S Testing, BB Antibody Titer, Platelet Antibody/Crossmatch, HLA Typing, Volume Reduction, CMV Required)

*Transfusion Reaction Testing may include all testing above  and TX Reaction Workup, Blood Culture/Gram Stain, Urine Hemoglobin, TRALI Investigation.
Administrative

CPT Codes

86900, 86901

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