Separate serum or plasma from whole blood within 24 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature. Transfer plasma to a sterile polypropylene tube.
Unacceptable Conditions
Heparinized specimens.
Storage/Transport Temperature
Refrigerated.
Stability (from collection to initiation)
Serum and plasma specimens may be stored at room temperature for up to 3 days, at 2-8°C for up to 7 days or frozen at -20°C to -80°C for up to six weeks.
Serum or plasma specimens may be frozen and thawed up to five times without a loss of HBV DNA.
Cerner Orderable(s)
Hepatitis B PCR Viral Load
Performed
Weekly
Ordering Recommendations
Detect and quantify hepatitis B virus.
Methodology
Quantitative Polymerase Chain Reaction
Reported
Weekly
Synonyms
HBV Quant PCR
HBV quantitative
HBV Viral Load
Hep B DNA
Reference Interval
Not detected
Interpretive Data
Linear range of assay: 20 IU/mL (1.3 log) - 170,000,000 IU/mL (8.2 log). This assay was performed with the FDA-approved Roche COBAS
CPT Codes
87517
Collection
Patient Preparation
No special patient preparation is necessary.
Collect
Human serum or plasma isolated from whole blood collected in EDTA (purple top) tubes or BD SST Serum Separator Tubes.
Separate serum or plasma from whole blood within 24 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature. Transfer plasma to a sterile polypropylene tube.
Unacceptable Conditions
Heparinized specimens.
Storage/Transport Temperature
Refrigerated.
Stability (from collection to initiation)
Serum and plasma specimens may be stored at room temperature for up to 3 days, at 2-8°C for up to 7 days or frozen at -20°C to -80°C for up to six weeks.
Serum or plasma specimens may be frozen and thawed up to five times without a loss of HBV DNA.
Ordering
Cerner Orderable(s)
Hepatitis B PCR Viral Load
Performed
Weekly
Ordering Recommendations
Detect and quantify hepatitis B virus.
Methodology
Quantitative Polymerase Chain Reaction
Reported
Weekly
Synonyms
HBV Quant PCR
HBV quantitative
HBV Viral Load
Hep B DNA
Result Interpretation
Reference Interval
Not detected
Interpretive Data
Linear range of assay: 20 IU/mL (1.3 log) - 170,000,000 IU/mL (8.2 log). This assay was performed with the FDA-approved Roche COBAS
