Evaluate parotitis and pancreatic disease. Amylase is primarily produced in the pancreas and salivary glands. Isoenzymes may be used to determine the source of an elevated amylase concentration. Measurement of pancreatic amylase activity is of value in diagnosing pancreatitis and other pancreatic disorders that result in the elevation of serum and urine amylase.
Collect
Gel-barrier tube, red-top tube, or green-top (heparin) tube. Separate serum or plasma from cells within 30 minutes of collection.
Specimen Preparation
If a red-top tube tube is used, transfer 2.0 mL separated serum to a plastic transport tube.
Unacceptable Conditions
Hemolysis, use of anticoagulants other than lithium heparin (Citrate and fluoride inhibit the assay reaction.)
Storage/Transport Temperature
Refrigerate
Stability (from collection to initiation)
After separation from cells: Room temperature: 7 days; Refrigerated: 1 month
Total amylase: enzymatic; pancreatic and salivary amylase: salivary amylase is inhibited by specific antibodies and the remaining pancreatic amylase is determined by an enzymatic, colorimetric method. Salivary amylase is calculated as the difference between the total and pancreatic amylase.
Reported
Report available: 4-5 days
Synonyms
AMYISO
Amylase Fractionation
Pancreatic Isoamylase
Reference Interval
See Laboratory Report
CPT Codes
82150 (x2)
Performing Lab
LabCorp Tampa
Collection
Purpose
Evaluate parotitis and pancreatic disease. Amylase is primarily produced in the pancreas and salivary glands. Isoenzymes may be used to determine the source of an elevated amylase concentration. Measurement of pancreatic amylase activity is of value in diagnosing pancreatitis and other pancreatic disorders that result in the elevation of serum and urine amylase.
Collect
Gel-barrier tube, red-top tube, or green-top (heparin) tube. Separate serum or plasma from cells within 30 minutes of collection.
Specimen Preparation
If a red-top tube tube is used, transfer 2.0 mL separated serum to a plastic transport tube.
Unacceptable Conditions
Hemolysis, use of anticoagulants other than lithium heparin (Citrate and fluoride inhibit the assay reaction.)
Storage/Transport Temperature
Refrigerate
Stability (from collection to initiation)
After separation from cells: Room temperature: 7 days; Refrigerated: 1 month
Total amylase: enzymatic; pancreatic and salivary amylase: salivary amylase is inhibited by specific antibodies and the remaining pancreatic amylase is determined by an enzymatic, colorimetric method. Salivary amylase is calculated as the difference between the total and pancreatic amylase.
