Ordering Recommendations

This qualitative NAAT is intended for the detection of SARS-CoV-2.

Reference respiratory virus communications for most recent information: Provider News and Publications

Collection Instructions

Nasopharyngeal swab method (preferred)
Use only synthetic fiber swabs with plastic shafts. Do not use calcium alginate swabs or swabs with wooden shafts, as they may contain substances that inhibit PCR testing.
1.) Carefully insert swab into the nostril parallel to the palate.
2.) Rotate swab over the surface of the posterior nasopharynx, keeping the swab in the nasopharynx for several seconds to absorb secretions.
3.) Slowly withdraw the swab from the collection site.
4.) Insert immediately into sterile tubes containing 3mL transport media. You will need to break off or cut the swab shaft to fit into the vial. 
5.) Securely close the vial.
6.) Label the vial with specimen identification label or hand label. Specimen must be labeled with last name, first name and date of birth.
7.) Place each vial in a separate, individual specimen transport bag. Seal the zip lock. Place folded paper requisition in the outer front pocket of the transport bag.

Nasal Swab Method
1.) Carefully insert the swab into the nostril that has the most visible drainage or that is the most congested if drainage is not visible. Swab should be inserted until resistance is met or approximately 2.5 cm into the nasal cavity.
2.) Gently rotate the swab 5 times around the nasal wall.
3.) Slowly remove from the nostril.
4.) Repeat process by carefully inserting into the second nostril. Swab should be inserted until resistance is met or approximately 2.5 cm into the nasal cavity. Gently rotate the swab 5 times around the nasal wall. Slowly remove from the nostril.
5.) Immediately place the swab in the vial with the transport medium. You will need to break off or cut the swab shaft to fit into the vial.
6.) Securely close the vial.
7.) Label vial with specimen identification label or hand label. Specimen must be labeled with last name, first name and date of birth.
8.) Place each vial in a separate, individual specimen transport bag. Seal the zip lock. Place folded paper requisition in the outer front pocket of the transport bag.

Nasopharyngeal wash/aspirate
1.) Collect 2-3mL in a sterile, leak-proof, screw-cap sputum collection cup or sterile dry container.
2.) Place each container in a separate, individual specimen transport bag. Seal the zip lock. Place the folded paper requisition in the outer front pocket of the transport bag.

Collect

Upper respiratory specimens: nasopharyngeal swab in UTM or nasal swab in UTM or pharyngeal swab in UTM.
Nasopharyngeal wash/aspirate also acceptable. (Tested at UR Central Lab)

Lower respiratory specimens: BAL, Bronchial Wash (Tested at UR Central Lab)

Specimen source required.  

Storage/Transport Temperature

Transport to laboratory immediately at ambient temperature or at 2-8 °C

DO NOT FREEZE.
 

Unacceptable Conditions

Specimens received outside of stability.
Specimens that leaked in transit. 
Unlabeled specimens. 
Mislabeled specimens or specimens having less that 2 identifiers. 
Specimens received in an inappropriate preservative or collection container. 
Wooden swabs. Dry swabs. 
Swabs not collected and placed in BD UTM. 
Frozen samples. 
Specimens submitted without a source. 
Unlabeled or expired UTM. 
Media other than BD UTM. 

Remarks

Testing for COVID-19 is available based on the provider’s clinical judgement. At a minimum, patients should have influenza-like illness or febrile lower respiratory infection. Tesing of asymptomatic or minimally symptomatic patients may be appropriate in some circumstances.
 

Stability (from collection to initiation)

Ambient (room temperature): 7 days (HH 48 hours)
Refrigerated (2-8 °C): 7 days

NOTE: Data collected by Central Labs has documented stability at ambient and refrigerated temperatures for up to 7 days.

Collection device

Universal Transport Media

 

Minimum Requirements

1.0 mL

Lab Section

Microbiology

Methodology

Reverse transcription Polymerase Chain Reaction 

Performed

Sun-Sat

Reported

1 day; possibly 2 days during periods of higher than normal test volumes

Performing Laboratory Website (click below)

UR Medicine Labs

Reference Interval

COVID-19 nucleic acid not detected

CPT Codes

87635

LOINC Mapping

94500-6

Order Type (Individual or Group)

G

Group Test Information

Result Test ID Reportable Result Test Name Result Type Type (Alpha or Numeric) Prompt Test
SRCCV Y COVID-19 SOURCE I A Y
COV19 Y COVID-19 PCR I A  

Prompt Test

SRCCV - Response Options (Case Sensititive)
1. Nasopharyngeal
2. Oropharyngeal
3. Nasal
4. Other

Micro Source Information

Nasopharyngeal

CPT Codes

87635

LOINC Mapping

94500-6

Pricing

Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu
Specimen Requirements

Ordering Recommendations

This qualitative NAAT is intended for the detection of SARS-CoV-2.

Reference respiratory virus communications for most recent information: Provider News and Publications

Collection Instructions

Nasopharyngeal swab method (preferred)
Use only synthetic fiber swabs with plastic shafts. Do not use calcium alginate swabs or swabs with wooden shafts, as they may contain substances that inhibit PCR testing.
1.) Carefully insert swab into the nostril parallel to the palate.
2.) Rotate swab over the surface of the posterior nasopharynx, keeping the swab in the nasopharynx for several seconds to absorb secretions.
3.) Slowly withdraw the swab from the collection site.
4.) Insert immediately into sterile tubes containing 3mL transport media. You will need to break off or cut the swab shaft to fit into the vial. 
5.) Securely close the vial.
6.) Label the vial with specimen identification label or hand label. Specimen must be labeled with last name, first name and date of birth.
7.) Place each vial in a separate, individual specimen transport bag. Seal the zip lock. Place folded paper requisition in the outer front pocket of the transport bag.

Nasal Swab Method
1.) Carefully insert the swab into the nostril that has the most visible drainage or that is the most congested if drainage is not visible. Swab should be inserted until resistance is met or approximately 2.5 cm into the nasal cavity.
2.) Gently rotate the swab 5 times around the nasal wall.
3.) Slowly remove from the nostril.
4.) Repeat process by carefully inserting into the second nostril. Swab should be inserted until resistance is met or approximately 2.5 cm into the nasal cavity. Gently rotate the swab 5 times around the nasal wall. Slowly remove from the nostril.
5.) Immediately place the swab in the vial with the transport medium. You will need to break off or cut the swab shaft to fit into the vial.
6.) Securely close the vial.
7.) Label vial with specimen identification label or hand label. Specimen must be labeled with last name, first name and date of birth.
8.) Place each vial in a separate, individual specimen transport bag. Seal the zip lock. Place folded paper requisition in the outer front pocket of the transport bag.

Nasopharyngeal wash/aspirate
1.) Collect 2-3mL in a sterile, leak-proof, screw-cap sputum collection cup or sterile dry container.
2.) Place each container in a separate, individual specimen transport bag. Seal the zip lock. Place the folded paper requisition in the outer front pocket of the transport bag.

Collect

Upper respiratory specimens: nasopharyngeal swab in UTM or nasal swab in UTM or pharyngeal swab in UTM.
Nasopharyngeal wash/aspirate also acceptable. (Tested at UR Central Lab)

Lower respiratory specimens: BAL, Bronchial Wash (Tested at UR Central Lab)

Specimen source required.  

Storage/Transport Temperature

Transport to laboratory immediately at ambient temperature or at 2-8 °C

DO NOT FREEZE.
 

Unacceptable Conditions

Specimens received outside of stability.
Specimens that leaked in transit. 
Unlabeled specimens. 
Mislabeled specimens or specimens having less that 2 identifiers. 
Specimens received in an inappropriate preservative or collection container. 
Wooden swabs. Dry swabs. 
Swabs not collected and placed in BD UTM. 
Frozen samples. 
Specimens submitted without a source. 
Unlabeled or expired UTM. 
Media other than BD UTM. 

Remarks

Testing for COVID-19 is available based on the provider’s clinical judgement. At a minimum, patients should have influenza-like illness or febrile lower respiratory infection. Tesing of asymptomatic or minimally symptomatic patients may be appropriate in some circumstances.
 

Stability (from collection to initiation)

Ambient (room temperature): 7 days (HH 48 hours)
Refrigerated (2-8 °C): 7 days

NOTE: Data collected by Central Labs has documented stability at ambient and refrigerated temperatures for up to 7 days.

Collection device

Universal Transport Media

 

Minimum Requirements

1.0 mL
Testing

Lab Section

Microbiology

Methodology

Reverse transcription Polymerase Chain Reaction 

Performed

Sun-Sat

Reported

1 day; possibly 2 days during periods of higher than normal test volumes

Performing Laboratory Website (click below)

UR Medicine Labs
Result Interpretation

Reference Interval

COVID-19 nucleic acid not detected
Coding

CPT Codes

87635

LOINC Mapping

94500-6
URM Labs Internal
Test Build

Order Type (Individual or Group)

G

Group Test Information

Result Test ID Reportable Result Test Name Result Type Type (Alpha or Numeric) Prompt Test
SRCCV Y COVID-19 SOURCE I A Y
COV19 Y COVID-19 PCR I A  

Prompt Test

SRCCV - Response Options (Case Sensititive)
1. Nasopharyngeal
2. Oropharyngeal
3. Nasal
4. Other

Micro Source Information

Nasopharyngeal

CPT Codes

87635

LOINC Mapping

94500-6

Pricing

Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu

Important Information

Lab Section
Microbiology

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