Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date. Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Refrigerated: 3 days; Frozen: > 3 days at -20°
Ordering Recommendations
This orderable is intended to aid in the initial diagnosis of a variety of diseases caused by autoantibodies, including systemic lupus erythematosus (SLE), Sjögren syndrome, mixed connective tissue disease (MCTD), scleroderma, polymyositis, and CREST syndrome. Following a digital ANA by IFA, subsequent orderables may automatically be reflexed covering anti-dsDNA, chromatin, ribosome P, and centromere antibodies, as well as antibodies against the extractable nuclear antigens SS-A/Ro, SS-B/La, Sm, RNP, Scl-70, and Jo 1 and also complement (C3/C4).
Performed
Monday - Friday; Days
Methodology
Varies on reflex testing; includes immunofluorescence, may include Multiplex Flow Immunoassay, fluorescence immunoassay, Rate Nephelometry, and EIA.
Reported
1-7 days
Performing Lab
Serology/Chemistry
Synonyms
ANA Ab IF QNw/Reflex
ANA Ab w/Reflex
ANA QN Cascade
11599
Reference Interval
ANA:
<1:80 - No detectable anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA)
1:80 - Indeterminate for anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA). Clinical-pathological correlation required; Approximately 13% of normal healthy individuals will test positive at the 1:80 dilution. A rheumatology consult may not be necessary.
>/= 1:160 Positive for anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA)
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date. Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Refrigerated: 3 days; Frozen: > 3 days at -20°
Ordering
Ordering Recommendations
This orderable is intended to aid in the initial diagnosis of a variety of diseases caused by autoantibodies, including systemic lupus erythematosus (SLE), Sjögren syndrome, mixed connective tissue disease (MCTD), scleroderma, polymyositis, and CREST syndrome. Following a digital ANA by IFA, subsequent orderables may automatically be reflexed covering anti-dsDNA, chromatin, ribosome P, and centromere antibodies, as well as antibodies against the extractable nuclear antigens SS-A/Ro, SS-B/La, Sm, RNP, Scl-70, and Jo 1 and also complement (C3/C4).
Performed
Monday - Friday; Days
Methodology
Varies on reflex testing; includes immunofluorescence, may include Multiplex Flow Immunoassay, fluorescence immunoassay, Rate Nephelometry, and EIA.
Reported
1-7 days
Performing Lab
Serology/Chemistry
Synonyms
ANA Ab IF QNw/Reflex
ANA Ab w/Reflex
ANA QN Cascade
11599
Result Interpretation
Reference Interval
ANA:
<1:80 - No detectable anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA)
1:80 - Indeterminate for anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA). Clinical-pathological correlation required; Approximately 13% of normal healthy individuals will test positive at the 1:80 dilution. A rheumatology consult may not be necessary.
>/= 1:160 Positive for anti-nuclear antibodies (ANA) by Digital Immunofluorescence Antibody Assay (IFA)
